UNASSIGNED: Human bone marrow-derived mesenchymal stromal cells (hBMSCs) were used for exosome isolation. Transmission electron microscopy (TEM) and confocal laser scanning microscopy were used for exosome and MDs morphological characterization, respectively. The MDs-NFATc1/PLA-Exo scaffold was fabricated through poly(dopamine) and fibrin gel coating. Biocompatibility was assessed using RAW 264.7 macrophages and hBMSCs. Osteoclast formations were examined via TRAP staining. Osteogenic differentiation of hBMSCs and cytokine expression modulation were also investigated.
UNASSIGNED: MSC-Exo exhibited a cup-shaped structure and effective internalization into cells, while MDs displayed a spherical morphology with a well-defined core-shell structure. Following ultrasound stimulation, the internalization study demonstrated efficient delivery of bioactive MDs into recipient cells. Biocompatibility studies indicated no cytotoxicity of MDs-NFATc1/PLA-Exo scaffolds in RAW 264.7 macrophages and hBMSCs. Both MDs-NFATc1/PLA and MDs-NFATc1/PLA-Exo treatments significantly reduced osteoclast differentiation and formation. In addition, our results further indicated MDs-NFATc1/PLA-Exo scaffold significantly enhanced osteogenic differentiation of hBMSCs and modulated cytokine expression.
UNASSIGNED: These findings suggest that the bioactive MDs-NFATc1/PLA-Exo scaffold holds promise as an innovative structure for bone tissue regeneration. By specifically targeting osteoclast formation and promoting osteogenic differentiation, this hybrid scaffold may address key challenges in osteoporosis-related fractures.
■人骨髓来源的间充质基质细胞(hBMSC)用于外泌体分离。透射电子显微镜(TEM)和共聚焦激光扫描显微镜用于外泌体和MD的形态表征,分别。MDs-NFATc1/PLA-Exo支架通过聚(多巴胺)和纤维蛋白凝胶涂层制备。使用RAW264.7巨噬细胞和hBMSC评估生物相容性。通过TRAP染色检查破骨细胞形成。还研究了hBMSCs的成骨分化和细胞因子表达调节。
■MSC-Exo表现出杯状结构并有效地内化到细胞中,而MD表现出具有明确定义的核-壳结构的球形形态。超声刺激后,内化研究证明了生物活性MD有效递送到受体细胞中.生物相容性研究表明MDs-NFATc1/PLA-Exo支架在RAW264.7巨噬细胞和hBMSCs中没有细胞毒性。MDs-NFATc1/PLA和MDs-NFATc1/PLA-Exo处理均显着降低了破骨细胞的分化和形成。此外,我们的结果进一步表明MDs-NFATc1/PLA-Exo支架显着增强了hBMSCs的成骨分化并调节了细胞因子的表达。
■这些发现表明,生物活性MD-NFATc1/PLA-Exo支架有望成为骨组织再生的创新结构。通过特异性靶向破骨细胞形成和促进成骨分化,这种混合支架可能解决骨质疏松症相关骨折的关键挑战.