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Abstract：
Stellera chamaejasme (S. chamaejasme) is an important medicinal plant with heat-clearing, detoxifying, swelling and anti-inflammatory effects. At the same time, it is also one of the iconic plants of natural grassland degradation in northwest China, playing a key role in the invasion process. Plant endophytes live in healthy plant tissues and can synthesize substances needed for plant growth, induce disease resistance in host plants, and enhance plant resistance to environmental stress. Therefore, studying the root endophytes of S. chamaejasme is of great significance for mining beneficial microbial resources and biological prevention and control of S. chamaejasme. This study used Illumina MiSeq high-throughput sequencing technology to analyze the composition and diversity of endophytes in the roots of S. chamaejasme in different alpine grasslands (BGC, NMC and XGYZ) in Tibet. Research results show that the main phylum of endophytic fungi in the roots of S. chamaejasme in different regions is Ascomycota, and the main phyla of endophytic bacteria are Actinobacteria, Proteobacteria and Firmicutes (Bacteroidota). Overall, the endophyte diversity of the NMC samples was significantly higher than that of the other two sample sites. Principal coordinate analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA) results showed significant differences in the composition of endophytic bacterial and fungal communities among BGC, NMC and XGYZ samples. Co-occurrence network analysis of endophytes showed that there were positive correlations between fungi and some negative correlations between bacteria, and the co-occurrence network of bacteria was more complex than that of fungi. In short, this study provides a vital reference for further exploring and utilizing the endophyte resources of S. chamaejasme and an in-depth understanding of the ecological functions of S. chamaejasme endophytes.
摘要：
斯特拉·恰玛斯梅（S.chamaejasme）是一种重要的清热药用植物，解毒,肿胀和抗炎作用。同时,也是我国西北地区天然草地退化的标志性植物之一,在入侵过程中发挥关键作用。植物内生菌生活在健康的植物组织中，可以合成植物生长所需的物质，在寄主植物中诱导抗病性，增强植物对环境胁迫的抵抗力。因此,研究番荔枝的根系内生菌对挖掘有益微生物资源和防治番荔枝具有重要意义。本研究采用IlluminaMiSeq高通量测序技术，分析了不同高山草地的chamaejasme根中内生菌的组成和多样性（BGC，NMC和XGYZ）在西藏。研究结果表明,不同地区虎杖根中内生真菌的主要门系为子囊,内生细菌的主要门系是放线菌，变形杆菌和厚壁菌（拟杆菌）。总的来说,NMC样品的内生菌多样性显著高于其他两个样品位点。主坐标分析（PCoA）和置换多变量方差分析（PERMANOVA）结果表明，BGC之间的内生细菌和真菌群落组成存在显着差异，NMC和XGYZ样品。内生菌的共生网络分析显示,真菌之间存在正相关,细菌之间存在负相关,细菌的共现网络比真菌复杂。总之,本研究为进一步发掘和利用沙玛香内生菌资源以及深入了解沙玛香内生菌的生态功能提供了重要参考。
