Abstract：
Depression is a common mental disorder. In recent years, more and more attention has been paid to depression and its etiology and pathogenesis. This review aims to explore the neuroprotective and antidepressant effects of hop components. By establishing an in vitro cell damage model using PC12 cells induced by corticosterone (CORT) and an in vivo depression model through the intracranial injection of lipopolysaccharide (LPS) in mice, hop ethyl acetate extract (HEA) was used to study the protective effect and mechanism of HEA on neuronal cells in vitro and the antidepression effect and mechanism in vivo. The results showed that HEA increased the survival and decreased the rate of lactate dehydrogenase (LDH) release, apoptosis, and the ROS and NO content of CORT-induced PC12 cells. HEA alleviated depressive-like behavior, neuroinflammation, reduction of norepinephrine, and dendritic spines induced by intracerebroventricular injection of LPS in mice and increases the expression levels of BDNF, SNAP 25, and TrkB proteins without any significant side effects or toxicity. Hops demonstrated significant comprehensive utilization value, and this work provided an experimental basis for the role of hops in the treatment of depression and provided a basis for the development of HEA for antidepressant drugs or dietary therapy products.
摘要：
抑郁症是一种常见的精神障碍。近年来,抑郁症及其病因病机越来越受到重视。本文旨在探讨啤酒花成分的神经保护和抗抑郁作用。通过建立皮质酮（CORT）诱导的PC12细胞体外细胞损伤模型和小鼠颅内注射脂多糖（LPS）体内抑郁模型，用啤酒花乙酸乙酯提取物（HEA）研究HEA对神经元细胞的保护作用和机制，以及体内抗抑郁作用和机制。结果表明，HEA增加了存活率，降低了乳酸脱氢酶（LDH）的释放速率，凋亡，以及CORT诱导的PC12细胞的ROS和NO含量。HEA缓解了抑郁样行为，神经炎症，去甲肾上腺素的减少，小鼠脑室内注射LPS诱导的树突棘和增加BDNF的表达水平,SNAP25和TrkB蛋白没有任何显著的副作用或毒性。啤酒花具有显著的综合利用价值,这项工作为啤酒花在治疗抑郁症中的作用提供了实验基础，并为开发用于抗抑郁药物或饮食治疗产品的HEA提供了依据。
