Abstract：
Peripheral T-cell lymphomas (PTCL) are morphologically and biologically heterogeneous and a subset expresses CD30, including anaplastic large cell lymphomas (ALCL) and a minority of PTCL, not otherwise specified (PTCL, NOS). ALCL with ALK translocations (ALCL, ALK+) are readily identified by routine diagnostic methods, but differentiating ALCL without ALK translocation (ALCL, ALK-) and PTCL, NOS expressing CD30 (PTCL CD30+) can be challenging. Furthermore, rare PTCL co-express CD30 and CD15 (PTCL CD30+CD15+); some resemble ALCL, ALK- while others resemble classic Hodgkin lymphoma. To explore the relationship between PTCL CD30+CD15+ and ALCL, ALK-, we analysed 19 cases of PTCL with CD30 expression, previously diagnosed as ALCL, ALK- (nine cases) and PTCL CD30+CD15+ (10 cases) for DUSP22/IRF4 rearrangements, coding RNA expression and selected transcriptome analysis using the NanoString nCounter gene expression analysis platform. Unsupervised clustering showed no clear segregation between ALCL, ALK- and PTCL CD30+CD15+. Three cases previously classified as PTCL CD30+CD15+ showed DUSP22/IRF4 rearrangements, favouring a diagnosis of ALCL, ALK-. Our results suggest that cases previously designated PTCL CD30+CD15+, likely fall within the spectrum of ALCL, ALK-; additionally, a subset of ALCL, ALK- with DUSP22/IRF4 rearrangement expresses CD15, consistent with previous reports and expands the immunophenotypic spectrum of this lymphoma subgroup.
摘要：
外周T细胞淋巴瘤（PTCL）在形态和生物学上是异质的，并且一个子集表达CD30，包括间变性大细胞淋巴瘤（ALCL）和少数PTCL，未指定(PTCL，NOS).具有ALK易位的ALCL（ALCL，ALK+)很容易通过常规诊断方法识别，但区分无ALK易位的ALCL(ALCL，ALK-)和PTCL，NOS表达CD30(PTCLCD30+)可能是具有挑战性的。此外，罕见的PTCL共表达CD30和CD15（PTCLCD30+CD15+）；有些类似ALCL，ALK-而其他类似于经典的霍奇金淋巴瘤。探讨PTCLCD30+CD15+与ALCL的关系,ALK-,我们分析了19例CD30表达的PTCL，以前诊断为ALCL，ALK-(9例)和PTCLCD30+CD15+(10例)用于DUSP22/IRF4重排,编码RNA表达和使用NanoStringnCounter基因表达分析平台选择的转录组分析。无监督聚类显示ALCL之间没有明显的隔离，ALK-和PTCLCD30+CD15+。先前分类为PTCLCD30+CD15+的3例显示DUSP22/IRF4重排，有利于ALCL的诊断，ALK-。我们的结果表明，以前指定为PTCLCD30+CD15+的病例，可能属于ALCL的范围，ALK-；另外，ALCL的一个子集，具有DUSP22/IRF4重排的ALK-表达CD15，与以前的报道一致，并扩展了该淋巴瘤亚组的免疫表型谱。
