PDF(Pubmed)
Abstract：
Chronic inflammatory diseases are considered the most significant cause of death worldwide. Current treatments for inflammatory diseases are limited due to the lack of understanding of the biological factors involved in early-stage disease progression. Nerve growth factor (NGF) is a neurotrophic factor directly associated with inflammatory and autoimmune diseases like osteoarthritis, multiple sclerosis, and rheumatoid arthritis. It has been shown that NGF levels are significantly upregulated at the site of inflammation and play a crucial role in developing a robust inflammatory response. However, little is known about NGF\'s temporal expression profile during the initial progressive phase of inflammation. This study aimed to determine the temporal expression patterns of NGF in rat skin (epidermis) during adjuvant-induced arthritis (AIA). Sprague Dawley rats were randomly divided into control and complete Freund\'s adjuvant (CFA)-treated groups. Levels of NGF were evaluated following unilateral AIA at different time points, and it was found that peripheral inflammation due to AIA significantly upregulated the expression of NGF mRNA and protein in a biphasic pattern. These results suggest that NGF signaling is crucial for initiating and maintaining peripheral neurogenic inflammation in rats during AIA.
摘要：
慢性炎性疾病被认为是世界上最重要的死亡原因。由于缺乏对早期疾病进展中涉及的生物学因素的了解,目前对炎性疾病的治疗是有限的。神经生长因子(nervegrowthfactor,NGF)是一种与骨关节炎等炎症和自身免疫性疾病直接相关的神经营养因子。多发性硬化症,和类风湿性关节炎。已经显示,NGF水平在炎症部位显著上调,并且在发展强烈的炎症反应中起关键作用。然而,在炎症的初始进展阶段，对NGF的时间表达谱知之甚少。本研究旨在确定佐剂性关节炎（AIA）期间大鼠皮肤（表皮）中NGF的时间表达模式。将SD大鼠随机分为对照组和完全弗氏佐剂(CFA)治疗组。在不同时间点的单侧AIA后评估NGF水平，发现AIA引起的外周炎症以双相模式显著上调NGFmRNA和蛋白的表达。这些结果表明，在AIA期间，NGF信号传导对于启动和维持大鼠周围神经源性炎症至关重要。
