PDF(Pubmed)
Abstract：
Vitis vinifera L. possesses high economic value, but its growth and yield are seriously affected by salt stress. Though melatonin (MT) has been widely reported to enhance tolerance towards abiotic stresses in plants, the regulatory role melatonin plays in resisting salt tolerance in grapevines has scarcely been studied. Here, we observed the phenotypes under the treatment of different melatonin concentrations, and then transcriptome and metabolome analyses were performed. A total of 457 metabolites were detected in CK- and MT-treated cell cultures at 1 WAT (week after treatment) and 4 WATs. Exogenous melatonin treatment significantly increased the endogenous melatonin content while down-regulating the flavonoid content. To be specific, the melatonin content was obviously up-regulated, while the contents of more than a dozen flavonoids were down-regulated. Auxin response genes and melatonin synthesis-related genes were regulated by the exogenous melatonin treatment. WGCNA (weighted gene coexpression network analysis) identified key salt-responsive genes; they were directly or indirectly involved in melatonin synthesis and auxin response. The synergistic effect of salt and melatonin treatment was investigated by transcriptome analysis, providing additional evidence for the stress-alleviating properties of melatonin through auxin-related pathways. The present study explored the impact of exogenous melatonin on grapevines\' ability to adapt to salt stress and provided novel insights into enhancing their tolerance to salt stress.
摘要：
葡萄具有很高的经济价值，但其生长和产量受到盐胁迫的严重影响。尽管褪黑激素（MT）已被广泛报道可增强植物对非生物胁迫的耐受性，褪黑素在葡萄耐盐性中的调节作用几乎没有研究。这里,我们观察了不同褪黑素浓度处理下的表型，然后进行转录组和代谢组分析。在1个WAT（处理后一周）和4个WATs的CK和MT处理的细胞培养物中检测到总共457种代谢物。外源性褪黑素处理显著增加内源性褪黑素含量,同时下调黄酮含量。具体而言,褪黑素含量明显上调,而十几种类黄酮的含量下调。生长素应答基因和褪黑素合成相关基因受外源性褪黑素处理调控。WGCNA（加权基因共表达网络分析）确定了关键的盐反应基因；它们直接或间接参与褪黑激素的合成和生长素反应。通过转录组分析研究了盐和褪黑激素治疗的协同作用，为褪黑激素通过生长素相关途径缓解应激的特性提供了额外的证据。本研究探讨了外源褪黑素对葡萄适应盐胁迫能力的影响，并为增强葡萄对盐胁迫的耐受性提供了新的见解。
