PDF(Pubmed)
Abstract：
Human noroviruses are major causes of foodborne outbreaks linked to berries. The overall goal of this study was to investigate the persistence of a human norovirus surrogate, Tulane virus (TV), in berry smoothies and under simulated digestion through the gastrointestinal track. Two types of smoothies were prepared from blueberries and strawberries. Tulane virus was spiked into each smoothie and incubated either at 37 or 4 °C for 2, 60, and 120 min. Furthermore, the virus-spiked smoothies were subjected to sequential oral (2 min), gastric (10 and 60 min), and intestinal (15 and 120 min) digestion according to the standardized INFOGEST model. Quantification of infectious TV was carried out using the TCID50 assay. At 4 °C, in both berry smoothies, TV infectivity did not show significant changes throughout the 120 min period. At 37 °C, TV infectivity showed significant reduction (~0.5 log TCID50/mL) only in blueberry smoothies starting at 60 min. During the oral, gastric, and intestinal digestion phases, the mean log reduction in TV infectivity in blueberry did not exceed ~0.5 log, while infectious TV in strawberry smoothies under all phases was stable. Given the notable stability of infectious viruses in berry smoothies and the gastrointestinal tract, prevention of norovirus contamination of berries is paramount to reduce virus outbreaks linked to berries.
摘要：
人类诺如病毒是与浆果有关的食源性疾病爆发的主要原因。这项研究的总体目标是调查人类诺如病毒替代品的持久性，杜兰病毒(电视),在浆果冰沙和通过胃肠道模拟消化。由蓝莓和草莓制备两种类型的冰沙。将杜兰病毒掺入每个冰沙中并在37或4°C下孵育2、60和120分钟。此外，将掺入病毒的冰沙连续口服（2分钟），胃（10和60分钟），和肠道消化（15和120分钟）根据标准化的INFOGEST模型。使用TCID50测定法进行感染性TV的定量。在4°C时,在两种浆果冰沙中，在整个120分钟内，电视的感染力没有显着变化。在37°C时，从60分钟开始，仅在蓝莓冰沙中，TV感染力显着降低（〜0.5logTCID50/mL）。在口头,胃,和肠道消化阶段，蓝莓中电视感染力的平均对数减少不超过~0.5对数，而在所有阶段的草莓冰沙中的传染性电视是稳定的。鉴于浆果冰沙和胃肠道中感染性病毒的显着稳定性，预防诺如病毒对浆果的污染对于减少与浆果有关的病毒爆发至关重要。
