PDF(Pubmed)
Abstract：
Fish gelatin (FG)-based wound dressings exhibit superior water absorption capacity, thermal stability, and gelation properties, which enhance the performance of these dressings. In this study, our objective was to investigate the conditions underlying the enzymatic hydrolysis of FG and subsequent cross-linking to prepare high-performance gels. A two-step enzymatic method of protease-catalyzed hydrolysis followed by glutamine transglutaminase (TGase)-catalyzed cross-linking was used to prepare novel high-performance fish gelatin derivatives with more stable dispersion characteristics than those of natural gelatin derivatives. Compared with conventional TGase cross-linked derivatives, the novel derivatives were characterized by an average pore size of 150 μm and increased water solubility (423.06% to 915.55%), water retention (by 3.6-fold to 43.89%), thermal stability (from 313 °C to 323 °C), and water vapor transmission rate, which reached 486.72 g·m-2·24 h-1. In addition, loading glucose oxidase onto the fish gelatin derivatives increased their antibacterial efficacy to >99% against Escherichia coli and Staphylococcus aureus.
摘要：
基于鱼明胶（FG）的伤口敷料表现出优异的吸水能力，热稳定性，和凝胶化特性，这提高了这些敷料的性能。在这项研究中,我们的目标是研究FG的酶促水解和随后的交联以制备高性能凝胶的基础条件。采用蛋白酶催化水解，然后进行谷氨酰胺转谷氨酰胺酶（TGase）催化交联的两步酶法，制备了新型高效鱼明胶衍生物，其分散特性比天然明胶衍生物更稳定。与传统的TGase交联衍生物相比，新型衍生物的特征是平均孔径为150μm，水溶性增加（423.06%至915.55%），保水性（增长3.6倍至43.89%），热稳定性（从313°C到323°C），和水蒸气透过率，达到486.72g·m-2·24h-1。此外,将葡萄糖氧化酶加载到鱼明胶衍生物上可将其对大肠杆菌和金黄色葡萄球菌的抗菌功效提高到>99％。
