PDF(Pubmed)
Abstract：
The relationship of PAX1/JAM3 methylation as well as HPV viral load (VL) with cervical lesions has been reported, but their role in persistent HPV infection without cervical high-grade lesions has not been fully elucidated. A total of 231 females diagnosed with persistent HPV infection and pathologically confirmed absence of high-grade cervical lesions were selected from the Colposcopy Outpatient Clinic of Peking University People\'s Hospital, from March 2023 to December 2023. They were categorized into two groups based on the duration of HPV infection: the HPV persistent less than 3 years group and the more than 3 years group. PAX1/JAM3 methylation and HPV VL were determined by real-time PCR and BioPerfectus Multiplex Real-Time (BMRT)-HPV reports type-specific VL/10,000 cells, respectively. The average age of individuals with HPV infection lasting more than 3 years was higher compared to those with less than 3 years (48.9 vs. 45.1 years), with a statistically significant difference. Among the participants, 81.8% (189/231) had no previous screening. The methylation levels of JAM3 and PAX1 were significantly higher in individuals with HPV infection persisting for more than 3 years compared to those with less than 3 years, with a statistically significant difference (p < 0.05). There was a significant correlation between PAX1 and JAM3 methylation (p < 0.001), which could be used as cumulative evidence of HPV infection duration before the occurrence of precancerous lesions. The incidence of vaginal intraepithelial lesions was higher in individuals with HPV infection persisting for more than 3 years compared to those with less than 3 years, and HPV VL can be used as an indicative biomarker for concurrent cervical-vaginal lesions, especially for HPV other than 16/18 genotypes.
摘要：
PAX1/JAM3甲基化以及HPV病毒载量(VL)与宫颈病变的关系已有报道,但其在无宫颈高级别病变的持续性HPV感染中的作用尚未完全阐明.选取北京大学人民医院阴道镜门诊确诊为持续性HPV感染且经病理证实无高级别宫颈病变的女性231例，从2023年3月到2023年12月。根据HPV感染的持续时间将其分为两组：HPV持续少于3年组和超过3年组。通过实时PCR和BioPerfectusMultipleReal-Time（BMRT）-HPV报告类型特异性VL/10,000细胞确定PAX1/JAM3甲基化和HPVVL，分别。HPV感染持续时间超过3年的个体的平均年龄高于小于3年的个体（48.9岁vs.45.1年)，具有统计学上的显著差异。在参与者中，81.8%(189/231)没有既往筛查。与HPV感染持续3年以上的个体相比，JAM3和PAX1的甲基化水平显着高于少于3年的个体。差异有统计学意义(p<0.05)。PAX1和JAM3甲基化之间存在显著相关性(p<0.001),可作为癌前病变发生前HPV感染持续时间的累积证据。与HPV感染持续3年以上的个体相比，阴道上皮内病变的发生率较高。和HPVVL可以用作并发宫颈-阴道病变的指示性生物标志物，特别是对于16/18基因型以外的HPV。
