Abstract：
Linusorbs (LO), cyclolinopeptides, are a group of cyclic hydrophobic peptides and considered a valuable by-product of flaxseed oil due to numerous health benefits. Currently applied acetone or methanol extraction could contaminate the feedstocks for further food-grade application. Using flaxseed cake as feedstock, this study established a practical method for preparing LO from pressed cake. Firstly, LO composition of 15 flaxseed cultivars was analyzed. Next, cold-pressed cake was milled and screened mechanically. The kernel and hull fractions were separated based on the disparity of their mechanical strength. Monitored by hyperspectral fluorescence, the LO-enriched kernel fraction separated from cold-pressed flaxseed cake was further used as feedstock for LO production. After ethanol extraction, partition, and precipitation, LOs were extracted from cold-pressed flaxseed cake with a purity of 91.4%. The proposed method could serve as feasible flaxseed cake valorization strategy and enable the preparation of other polar compounds such as flax lignan and mucilage.
摘要：
Linusorbs(LO)，环缩肽,是一组环状疏水肽，由于许多健康益处，被认为是亚麻籽油的有价值的副产品。目前应用的丙酮或甲醇萃取可能会污染用于进一步食品级应用的原料。以亚麻籽饼为原料,本研究建立了从压榨饼制备LO的实用方法。首先,分析了15个亚麻籽品种的LO成分。接下来,将冷压饼研磨并机械筛分。根据机械强度的差异，将内核和船体部分分开。通过高光谱荧光监测，从冷压亚麻籽饼中分离出的富含LO的籽粒部分进一步用作LO生产的原料。乙醇提取后，分区,和降水,从冷榨亚麻籽饼中提取LOs，纯度为91.4％。所提出的方法可以作为可行的亚麻籽饼增值策略，并能够制备其他极性化合物，例如亚麻木酚素和粘液。
