PDF(Pubmed)
Abstract：
A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole-genome sequencing, real-time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody-based immunoassay techniques, use of paper-based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats-Cas system-based technology, which can also be deployed for the detection of SARS-CoV-2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID-19.
摘要：
最近爆发的新型冠状病毒，COVID-19，在武汉市，中国,2019年12月，其随后在中国境内和各地的传播已导致数人死亡和感染。目前,核酸扩增检测对于确认COVID感染至关重要。在这份报告中,我们总结了六种有前途的方法，包括全基因组测序，实时逆转录聚合酶链反应，纳米孔靶测序，基于抗体的免疫测定技术，使用纸质生物分子传感器，以及基于Cas系统的成簇规则间隔短回文重复序列技术，它也可以用于检测SARS-CoV-2。我们进一步介绍了这些方法的原理，讨论了现有产品和方法的应用范围和实用性，并强调了开发用于COVID-19早期诊断的其他产品和技术的潜在方法。
