PDF(Pubmed)
Abstract：
Glioblastoma is the most frequent and aggressive brain tumor in adults. This study aims to evaluate the expression and prognostic impact of CD99, a membrane glycoprotein involved in cellular migration and invasion. In a cohort of patients with glioblastoma treated with surgery, radiotherapy and temozolomide, we retrospectively analyzed tumor expression of CD99 by immunohistochemistry (IHC) and by quantitative real-time polymerase chain reaction (qRT-PCR) for both the wild type (CD99wt) and the truncated (CD99sh) isoforms. The impact on overall survival (OS) was assessed with the Kaplan-Meier method and log-rank test and by multivariable Cox regression. Forty-six patients with glioblastoma entered this study. Immunohistochemical expression of CD99 was present in 83%. Only the CD99wt isoform was detected by qRT-PCR and was significantly correlated with CD99 expression evaluated by IHC (rho = 0.309, p = 0.037). CD99 expression was not associated with OS, regardless of the assessment methodology used (p = 0.61 for qRT-PCR and p = 0.73 for IHC). In an exploratory analysis of The Cancer Genome Atlas, casuistry of glioblastomas CD99 expression was not associated with OS nor with progression-free survival. This study confirms a high expression of CD99 in glioblastoma but does not show any significant impact on survival. Further preclinical studies are needed to define its role as a therapeutic target in glioblastoma.
摘要：
胶质母细胞瘤是成人中最常见和侵袭性的脑肿瘤。这项研究旨在评估CD99的表达和预后影响，CD99是一种参与细胞迁移和侵袭的膜糖蛋白。在接受手术治疗的胶质母细胞瘤患者队列中，放疗和替莫唑胺，我们通过免疫组织化学(IHC)和定量实时聚合酶链反应(qRT-PCR)对野生型(CD99wt)和截短亚型(CD99sh)进行回顾性分析.使用Kaplan-Meier方法和对数秩检验以及多变量Cox回归评估对总生存期（OS）的影响。46例胶质母细胞瘤患者进入本研究。CD99的免疫组织化学表达占83％。通过qRT-PCR仅检测到CD99wt同种型，并且与通过IHC评估的CD99表达显着相关（rho=0.309，p=0.037）。CD99表达与OS无关，无论使用何种评估方法(qRT-PCRp=0.61,IHCp=0.73).在对癌症基因组图谱的探索性分析中，胶质母细胞瘤的诊断CD99表达与OS或无进展生存期无关。该研究证实了CD99在成胶质细胞瘤中的高表达,但未显示对存活的任何显著影响。需要进一步的临床前研究来确定其作为胶质母细胞瘤治疗靶点的作用。
