PDF(Pubmed)
Abstract：
Rodents have been confirmed as hosts of various vector-borne zoonotic pathogens and are important for the maintenance of these microbes in nature. However, surveillance for zoonotic pathogens is limited for many wild rodent species in China, so our knowledge of pathogen ecology, genetic diversity, and the risk of cross-species transmission to humans is limited. In this study, 165 spleen samples of Daurian ground squirrels (Spermophilus dauricus) were collected from Weichang Manchu and the Mongolian Autonomous County of Hebei Province, China, and Rickettsia, Bartonella, and Anaplasma were identified by DNA detection using polymerase chain reaction (PCR). Sequence analysis identified eight bacterial pathogens: R. raoultii, R. sibirica, Candidatus R. longicornii, B. washoensis, B. grahamii, B. jaculi, A. capra, and Candidatus Anaplasma cinensis. Co-infection of B. grahamii and R. raoultii in one sample was observed. Our results demonstrated the genetic diversity of bacteria in Daurian ground squirrels and contributed to the distribution of these pathogens. Six species, A. capra, R. raoultii, R. sibirica, Candidatus R. longicornii, B. washoensis, and B. grahamii, are known to be pathogenic to humans, indicating a potential public health risk to the local human population, especially to herders who frequently have close contact with Daurian ground squirrels and are thus exposed to their ectoparasites.
摘要：
啮齿动物已被确认为各种媒介传播的人畜共患病原体的宿主，并且对于维持自然界中的这些微生物很重要。然而,在中国，对许多野生啮齿动物的人畜共患病原体的监测是有限的，所以我们对病原体生态学的了解，遗传多样性,跨物种传播给人类的风险是有限的。在这项研究中,从河北省围场满族和蒙古族自治县采集了165只Daurian地松鼠（Spermophilusdauricus）的脾脏样本，中国,还有立克次体,巴尔通体,使用聚合酶链反应（PCR）通过DNA检测鉴定无性体。序列分析确定了八种细菌病原体：R.raoultii，R.Sibirica,CandidatusR.longicornii,B.washoensis，B.grahamii,B.Jaculi,A.卡普拉,和念珠菌。在一个样品中观察到grahamii芽孢杆菌和raoultii的共感染。我们的结果表明了Daurian地松鼠中细菌的遗传多样性，并促成了这些病原体的分布。6种,A.卡普拉,R.Raoultii,R.Sibirica,CandidatusR.longicornii,B.washoensis，还有B.Grahamii,已知对人类有致病性，表明对当地人口的潜在公共卫生风险，尤其是那些经常与Daurian地松鼠密切接触并因此暴露于其体外寄生虫的牧民。
