Abstract：
The application of bioengineering techniques for achieving bone regeneration in the oral environment is an increasingly prominent field. However, the clinical use of synthetic materials carries certain risks. The liquid phase of concentrated growth factor (LPCGF), as a biologically derived material, exhibits superior biocompatibility. In this study, LPCGF was employed as a tissue engineering scaffold, hosting dental follicle cells (DFCs) to facilitate bone regeneration. Both in vivo and in vitro experimental results demonstrate that this platform significantly enhances the expression of osteogenic markers in DFCs, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and type I collagen (Col1a1). Simultaneously, it reduces the expression of inflammation-related genes, particularly interleukin-6 (IL-6) and interleukin-8 (IL-8), thereby alleviating the negative impact of the inflammatory microenvironment on DFCs. Further investigation into potential mechanisms reveals that this process is regulated over time by the WNT pathway. Our research results demonstrate that LPCGF, with its favorable physical characteristics, holds great potential as a scaffold. It can effectively carry DFCs, thereby providing an optimal initial environment for bone regeneration. Furthermore, LPCGF endeavors to closely mimic the mechanisms of bone healing post-trauma to facilitate bone formation. This offers new perspectives and insights into bone regeneration engineering.
摘要：
应用生物工程技术在口腔环境中实现骨再生是一个日益突出的领域。然而,合成材料的临床使用存在一定的风险。浓缩生长因子（LPCGF）的液相，作为一种生物衍生材料，具有优越的生物相容性。在这项研究中,LPCGF被用作组织工程支架，托管牙囊细胞（DFCs）以促进骨再生。体内和体外实验结果表明，该平台显着增强了DFCs中成骨标志物的表达，如碱性磷酸酶（ALP），runt相关转录因子2（Runx2），和I型胶原蛋白（Col1a1）。同时,它减少了炎症相关基因的表达，特别是白细胞介素-6（IL-6）和白细胞介素-8（IL-8），从而减轻炎症微环境对DFCs的负面影响。对潜在机制的进一步研究表明，该过程随时间受到WNT途径的调节。我们的研究结果表明，LPCGF，凭借其良好的物理特性，作为脚手架拥有巨大的潜力。它可以有效地携带DFCs，从而为骨骼再生提供最佳的初始环境。此外，LPCGF努力紧密地模拟创伤后骨愈合的机制以促进骨形成。这为骨再生工程提供了新的视角和见解。
