PDF(Pubmed)
Abstract：
BACKGROUND: Candida, a common oral microbiota, can cause opportunistic fungal infections. With rising Candida infections and limited effective antifungals, new treatments are needed. This study investigates carvacrol essential oil\'s effect on oral candidiasis, alone and with nystatin, compared to nystatin alone.
METHODS: In this study, oral samples were collected from dental clinic patients, especially denture users. The presence of Candida was confirmed and cultured from these samples. Candidiasis was detected by observing Candida colonies. Drug sensitivity was tested on 100 positive samples. The minimum concentration of inhibition and lethality of each isolate was evaluated using nystatin and carvacrol. The results were compared using two-way analysis of variance. Finally, the minimum inhibitory concentration (MIC) of nystatin and carvacrol was calculated individually and in combination.
RESULTS: The present study found that Candida albicans and non-albicans species were equally prevalent. Carvacrol showed significant biological activity against all Candida species, with an average MTT of 50.01%. The average MIC value of carvacrol was 24.96 µg/ml, indicating its potential to inhibit Candida growth. The mean Minimum Fungicidal Concentration (MFC) value of carvacrol was 23.48 µg/ml, suggesting its effectiveness in killing the fungi.
CONCLUSIONS: The study\'s findings reveal that the MIC of carvacrol was significantly lower than that of nystatin and the combination of nystatin and carvacrol. This suggests that carvacrol holds potential as an effective herbal remedy for candidiasis.
METHODS: In this study, oral samples were collected from dental clinic patients, especially denture users. The presence of Candida was confirmed and cultured from these samples. Candidiasis was detected by observing Candida colonies. Drug sensitivity was tested on 100 positive samples. The minimum concentration of inhibition and lethality of each isolate was evaluated using nystatin and carvacrol. The results were compared using two-way analysis of variance. Finally, the minimum inhibitory concentration (MIC) of nystatin and carvacrol was calculated individually and in combination.
RESULTS: The present study found that Candida albicans and non-albicans species were equally prevalent. Carvacrol showed significant biological activity against all Candida species, with an average MTT of 50.01%. The average MIC value of carvacrol was 24.96 µg/ml, indicating its potential to inhibit Candida growth. The mean Minimum Fungicidal Concentration (MFC) value of carvacrol was 23.48 µg/ml, suggesting its effectiveness in killing the fungi.
CONCLUSIONS: The study\'s findings reveal that the MIC of carvacrol was significantly lower than that of nystatin and the combination of nystatin and carvacrol. This suggests that carvacrol holds potential as an effective herbal remedy for candidiasis.
摘要：
背景：念珠菌，常见的口腔微生物群，会导致机会性真菌感染。随着念珠菌感染的增加和有效抗真菌药的减少，需要新的治疗方法。本研究调查香芹酚精油对口腔念珠菌病的影响，单独和制霉菌素，与单独的制霉菌素相比。
方法：在本研究中，口腔样本是从牙科诊所患者那里收集的，尤其是假牙使用者.从这些样品中确认并培养念珠菌的存在。通过观察念珠菌菌落检测到念珠菌病。在100个阳性样品上测试药物敏感性。使用制霉菌素和香芹酚评估每种分离物的最小抑制浓度和致死性。使用双向方差分析比较结果。最后,制霉菌素和香芹酚的最小抑制浓度(MIC)分别和联合计算.
结果：本研究发现白色念珠菌和非白色念珠菌同样普遍。香芹酚对所有念珠菌均显示出显着的生物活性，平均MTT为50.01%。香芹酚的平均MIC值为24.96µg/ml，表明其抑制念珠菌生长的潜力。香芹酚的平均最小杀菌浓度（MFC）值为23.48µg/ml，表明它在杀死真菌方面的有效性。
结论：研究结果表明，香芹酚的MIC明显低于制霉菌素和制霉菌素与香芹酚的组合。这表明香芹酚具有作为念珠菌病有效草药的潜力。
方法：在本研究中，口腔样本是从牙科诊所患者那里收集的，尤其是假牙使用者.从这些样品中确认并培养念珠菌的存在。通过观察念珠菌菌落检测到念珠菌病。在100个阳性样品上测试药物敏感性。使用制霉菌素和香芹酚评估每种分离物的最小抑制浓度和致死性。使用双向方差分析比较结果。最后,制霉菌素和香芹酚的最小抑制浓度(MIC)分别和联合计算.
结果：本研究发现白色念珠菌和非白色念珠菌同样普遍。香芹酚对所有念珠菌均显示出显着的生物活性，平均MTT为50.01%。香芹酚的平均MIC值为24.96µg/ml，表明其抑制念珠菌生长的潜力。香芹酚的平均最小杀菌浓度（MFC）值为23.48µg/ml，表明它在杀死真菌方面的有效性。
结论：研究结果表明，香芹酚的MIC明显低于制霉菌素和制霉菌素与香芹酚的组合。这表明香芹酚具有作为念珠菌病有效草药的潜力。
