Abstract：
Evolutionarily conserved protein associated with topoisomerase II (PAT1) proteins activate mRNA decay through binding mRNA and recruiting decapping factors to optimize posttranscriptional reprogramming. Here, we generated multiple mutants of pat1, pat1 homolog 1 (path1), and pat1 homolog 2 (path2) and discovered that pat triple mutants exhibit extremely stunted growth and all mutants with pat1 exhibit leaf serration while mutants with pat1 and path1 display short petioles. All three PATs can be found localized to processing bodies and all PATs can target ASYMMETRIC LEAVES 2-LIKE 9 transcripts for decay to finely regulate apical hook and lateral root development. In conclusion, PATs exhibit both specific and redundant functions during different plant growth stages and our observations underpin the selective regulation of the mRNA decay machinery for proper development.
摘要：
与拓扑异构酶II（PAT1）蛋白相关的进化保守蛋白通过结合mRNA和募集去盖因子来激活mRNA衰变，以优化转录后重编程。这里,我们产生了pat1，pat1同源物1（path1），和pat1同系物2（path2），并发现pat三重突变体表现出极度发育迟缓，所有pat1突变体表现出叶锯齿，而pat1和path1突变体表现出短叶柄。可以发现所有三个PAT都位于加工体，并且所有PAT都可以靶向不对称叶片2-LIKE9转录本进行衰变，以精细调节顶端钩和侧根发育。总之,PAT在不同的植物生长阶段表现出特定和冗余的功能，我们的观察结果为mRNA衰变机制的选择性调节提供了基础，以进行适当的发育。
