Abstract：
Membrane proteins play pivotal roles in a wide array of cellular processes and constitute approximately a quarter of the protein-coding genes across all organisms. Despite their ubiquity and biological significance, our understanding of these proteins remains notably less comprehensive compared to their soluble counterparts. This disparity in knowledge can be attributed, in part, to the inherent challenges associated with employing specialized techniques for the investigation of membrane protein insertion and topology. This review will center on a discussion of molecular biology methodologies and computational prediction tools designed to elucidate the insertion and topology of helical membrane proteins.
摘要：
膜蛋白在广泛的细胞过程中起着关键作用，并构成了所有生物体中大约四分之一的蛋白质编码基因。尽管它们无处不在，具有生物学意义，与可溶性蛋白质相比，我们对这些蛋白质的理解仍然不太全面。这种知识上的差距可以归因于，在某种程度上,与采用专门技术研究膜蛋白插入和拓扑结构相关的固有挑战。这篇综述将集中讨论分子生物学方法和旨在阐明螺旋膜蛋白的插入和拓扑结构的计算预测工具。
