Abstract：
As an important prognostic indicator in breast cancer, human epithelial growth factor receptor-2 (HER-2) is of importance for assessing prognosis of breast cancer patients, whose accurate and facile analysis are imperative in clinical diagnosis and treatment. Herein, photoactive Z-scheme UiO-66/CdIn2S4 heterojunction was constructed by a hydrothermal method, whose optical property and photoactivity were critically investigated by a range of techniques, combined by elucidating the interfacial charge transfer mechanism. Meanwhile, PtPdCu nanoflowers (NFs) were fabricated by a simple aqueous wet-chemical method, whose peroxidase (POD)-mimicking catalytic activity was scrutinized by representative tetramethylbenzidine (TMB) oxidation in H2O2 system. Taken together, the UiO-66/CdIn2S4 based photoelectrochemical (PEC) aptasensor was established for quantitative analysis of HER-2, where the detection signals were further magnified through catalytic precipitation reaction towards 4-chloro-1-naphthol (4-CN) oxidation (assisted by the PtPdCu NFs nanozyme). The PEC aptasensor presented a broader linear range within 0.1 pg mL-1-0.1 μg mL-1 and a lower limit of detection of 0.07 pg mL-1. This work developed a new PEC aptasensor for ultrasensitive determination of HER-2, holding substantial promise for clinical diagnostics.
摘要：
作为乳腺癌预后的重要指标,人上皮生长因子受体-2(HER-2)对评估乳腺癌患者的预后具有重要意义,其准确、简便的分析在临床诊断和治疗中势在必行。在这里，通过水热法构建了光活性Z方案UiO-66/CdIn2S4异质结，其光学性质和光活性通过一系列技术进行了严格的研究，结合阐明界面电荷转移机理。同时,PtPdCu纳米花（NFs）是通过简单的水性湿化学方法制备的，通过在H2O2系统中代表性的四甲基联苯胺（TMB）氧化来仔细检查其过氧化物酶（POD）模拟催化活性。一起来看,建立了基于UiO-66/CdIn2S4的光电化学（PEC）传感器，用于HER-2的定量分析，其中检测信号通过对4-氯-1-萘酚（4-CN）氧化的催化沉淀反应进一步放大（由PtPdCuNFs纳米酶辅助）。PECaptasensor在0.1pgmL-1-0.1μgmL-1范围内呈现较宽的线性范围，检测下限为0.07pgmL-1。这项工作开发了一种新的PECaptasensor，用于超灵敏地测定HER-2，对临床诊断具有实质性的希望。
