Abstract：
HSA (human serum albumin), a most abundant protein in blood serum, plays a key role in maintaining human health. Abnormal HSA level is correlated with many diseases, and thus has been used as an essential biomarker for therapeutic monitoring and biomedical diagnosis. Development of small-molecule fluorescent probes allowing the selective and sensitive recognition of HSA in in vitro and in vivo is of fundamental importance in basic biological research as well as medical diagnosis. Herein, we reported a series of new synthesized fluorescent dyes containing D-π-A constitution, which exhibited different optical properties in solution and solid state. Among them, dye M-H-SO3 with a hydrophilic sulfonate group at electron-acceptor part displayed selectivity for discrimination of HSA from BSA and other enzymes. Upon binding of dye M-H-SO3 with HSA, a significant fluorescence enhancement with a turn-on ratio about 96-fold was triggered. The detection limit was estimated to be ∼ 40 nM. Studies on the interaction mechanism revealed that dye M-H-SO3 could bind to site III of HSA with a 1:1 binding stoichiometry. Furthermore, dye M-H-SO3 has been applied to determine HSA in real urine samples with good recoveries, which provided a useful method for HSA analysis in biological fluids.
摘要：
HSA（人血清白蛋白），血清中最丰富的蛋白质，在维护人类健康中起着关键作用。HSA水平异常与多种疾病相关,因此已被用作治疗监测和生物医学诊断的重要生物标志物。开发允许在体外和体内选择性和灵敏识别HSA的小分子荧光探针在基础生物学研究以及医学诊断中具有根本重要性。在这里，我们报道了一系列新合成的含有D-π-A结构的荧光染料，在溶液和固体状态下表现出不同的光学性质。其中,在电子受体部分具有亲水性磺酸基的染料M-H-SO3显示出将HSA与BSA和其他酶区分开的选择性。染料M-H-SO3与HSA结合后，启动比约为96倍的显著荧光增强被触发.检测限估计为约40nM。对相互作用机理的研究表明，染料M-H-SO3可以以1：1的结合化学计量与HSA的位点III结合。此外，染料M-H-SO3已用于测定实际尿液样品中的HSA，具有良好的回收率，为生物流体中HSA的分析提供了一种有效的方法。
