Abstract：
Varicocele (VC) is considered as one of the primary causes of male infertility, and the pathogenesis and treatment measures for VC-associated male infertility (VMI) are being explored continuously. The construction of an appropriate in vitro model for VMI is of significant importance for the study of this disease. And the model of testis cell hypoxia, with its relatively stable experimental conditions, short cycle, good repeatability and few influencing factors, has been primarily applied in cellular experimental researches on VMI. This article reviews the selection of cell lines, use of modeling methods, and evaluation of cell models in recent VMI-related cellular experiments, aiming to provide some reference for scholars in their studies of the pathogenesis and treatment of VMI using the in vitro experimental model.
摘要：
精索静脉曲张（VC）被认为是男性不育的主要原因之一，VC相关性男性不育症(VMI)的发病机制和治疗措施正在不断探索中。构建合适的VMI体外模型对于该疾病的研究具有重要意义。和睾丸细胞缺氧的模型，其相对稳定的实验条件，短周期,重复性好，影响因素少，已主要应用于VMI的细胞实验研究。本文综述了细胞系的选择,使用建模方法，以及最近VMI相关细胞实验中细胞模型的评估，旨在为学者利用体外实验模型研究VMI的发病机制和治疗提供一定的参考。
