Abstract：
This study focused on studying the bioaccesible phenolic compounds (PCs) from yellow pea flour (F) and protein isolate (I). Total phenolic contents (TPC), PCs composition and antioxidant activities were analysed in ethanol 60% extracts obtained by applying ultrasound assisted extraction (UAE, 15 min/40% amplitude). The preparation of I under alkaline conditions and the elimination of some soluble components at lower pH produced a change of PCs profile and antioxidant activity. After simulated gastrointestinal digestion (SGID) of both ingredients to obtain the digests FD and ID, notable changes in the PCs concentration and profiles could be demonstrated. FD presented a higher ORAC activity than ID (IC50 = 0.022 and 0.039 mg GAE/g dm, respectively), but lower ABTS•+ activity (IC50 = 0.8 and 0.3 mg GAE/g dm, respectively). After treatment with cholestyramine of extracts from FD and ID in order to eliminate bile salts and obtain the bioaccesible fractions FDb and IDb, ROS scavenging in H2O2-induced Caco2-TC7 cells was evaluated, registering a greater activity for ID respect to FD (IC50 = 0.042 and 0.017 mg GAE/mL, respectively). These activities could be attributed to the major bioaccesible PCs: OH-tyrosol, polydatin, trans-resveratrol, rutin, (-)-epicatechin and (-)-gallocatechin gallate for FD; syringic (the most concentrated) and ellagic acids, trans-resveratrol, and (-)-gallocatechin gallate for ID, but probably other compounds such as peptides or amino acids can also contribute.
摘要：
这项研究的重点是研究黄色豌豆粉（F）和分离蛋白（I）中的生物可吸收酚类化合物（PC）。总酚含量（TPC），通过应用超声辅助提取（UAE，15min/40%振幅)。在碱性条件下制备I并在较低pH下消除某些可溶性成分会引起PCs分布和抗氧化活性的变化。经过两种成分的模拟胃肠消化（SGID）以获得消化物FD和ID，可以证明PCs浓度和轮廓的显着变化。FD表现出比ID更高的ORAC活性(IC50=0.022和0.039mgGAE/gdm，分别)，但ABTS•+活性较低(IC50=0.8和0.3mgGAE/gdm，分别)。在用来自FD和ID的提取物的消胆胺处理后，为了消除胆汁盐并获得生物可接受的部分FDb和IDb，评价H2O2诱导的Caco2-TC7细胞中的ROS清除,在FD方面记录更大的ID活性(IC50=0.042和0.017mgGAE/mL，分别)。这些活动可归因于主要的生物可访问的PC：OH-酪醇，虎杖苷,反式白藜芦醇，芦丁,FD的（-）-表儿茶素和（-）-gallocechin没食子酸酯；丁香酸（最浓）和鞣花酸，反式白藜芦醇，和(-)-gallocatechin没食子酸酯用于ID，但可能其他化合物如肽或氨基酸也有贡献。
