Abstract：
BACKGROUND: Traumatic spinal cord injury (SCI) is the most common preventable cause of morbidity. Despite rapid advances in medicine, effective pharmacological treatment against SCI has not yet been confirmed. This study aimed to investigate the possible anti-inflammatory, antiapoptotic, and neuroprotective effects of safinamide after SCI in a rat model.
METHODS: A total of 40 male Wistar albino rats were randomly divided into four groups. Group 1 underwent only laminectomy. Group 2 underwent SCI after laminectomy. In group 3, SCI was performed after laminectomy, and immediately afterward, intraperitoneal physiological saline solution was administered. In group 4, SCI was performed after laminectomy, and 90 mg/kg of safinamide was given intraperitoneally immediately afterward. Moderate spinal cord damage was induced at the level of thoracic vertebra nine (T9). Neuromotor function tests were performed and levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured. In both serum and spinal cord tissue, immunohistochemistry and histopathology studies were also conducted.
RESULTS: TNF-α, IL-1β, and IL-6 levels were found to be significantly increased in group 2 and group 3. In group 4, these levels were statistically significantly decreased. Group 4 also exhibited significant improvement in neuromotor function tests compared to the other groups. Histopathologically, it was found that group 4 showed significantly reduced inflammation and apoptosis compared to the other groups.
CONCLUSIONS: This study revealed that safinamide has neuroprotective effects against SCI due to its anti-inflammatory, antiapoptotic, and antioxidant activities.
METHODS: A total of 40 male Wistar albino rats were randomly divided into four groups. Group 1 underwent only laminectomy. Group 2 underwent SCI after laminectomy. In group 3, SCI was performed after laminectomy, and immediately afterward, intraperitoneal physiological saline solution was administered. In group 4, SCI was performed after laminectomy, and 90 mg/kg of safinamide was given intraperitoneally immediately afterward. Moderate spinal cord damage was induced at the level of thoracic vertebra nine (T9). Neuromotor function tests were performed and levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured. In both serum and spinal cord tissue, immunohistochemistry and histopathology studies were also conducted.
RESULTS: TNF-α, IL-1β, and IL-6 levels were found to be significantly increased in group 2 and group 3. In group 4, these levels were statistically significantly decreased. Group 4 also exhibited significant improvement in neuromotor function tests compared to the other groups. Histopathologically, it was found that group 4 showed significantly reduced inflammation and apoptosis compared to the other groups.
CONCLUSIONS: This study revealed that safinamide has neuroprotective effects against SCI due to its anti-inflammatory, antiapoptotic, and antioxidant activities.
摘要：
背景：创伤性脊髓损伤（SCI）是最常见的可预防的发病原因。尽管医学发展迅速，对SCI有效的药物治疗尚未得到证实。本研究旨在探讨可能的抗炎,抗凋亡，和safinamide在大鼠模型脊髓损伤后的神经保护作用。
方法：40只雄性Wistar白化病大鼠随机分为4组。第1组仅接受椎板切除术。第2组椎板切除术后接受SCI。在第3组中，在椎板切除术后进行SCI，紧接着，腹腔注射生理盐水。在第4组中，在椎板切除术后进行SCI，随后立即腹膜内给予90mg/kg沙芬酰胺。在胸椎九（T9）水平引起中度脊髓损伤。进行神经运动功能测试和肿瘤坏死因子-α（TNF-α）的水平，白细胞介素-6（IL-6），测定白细胞介素-1β(IL-1β)。在血清和脊髓组织中，还进行了免疫组织化学和组织病理学研究。
结果：TNF-α，IL-1β,发现第2组和第3组的IL-6水平显着增加。在第4组中，这些水平在统计学上明显下降。与其他组相比，第4组的神经运动功能测试也有显着改善。组织病理学,发现与其他组相比，第4组显示出显着减少的炎症和细胞凋亡。
结论：这项研究表明，沙芬酰胺具有抗SCI的神经保护作用，抗凋亡，和抗氧化活性。
方法：40只雄性Wistar白化病大鼠随机分为4组。第1组仅接受椎板切除术。第2组椎板切除术后接受SCI。在第3组中，在椎板切除术后进行SCI，紧接着，腹腔注射生理盐水。在第4组中，在椎板切除术后进行SCI，随后立即腹膜内给予90mg/kg沙芬酰胺。在胸椎九（T9）水平引起中度脊髓损伤。进行神经运动功能测试和肿瘤坏死因子-α（TNF-α）的水平，白细胞介素-6（IL-6），测定白细胞介素-1β(IL-1β)。在血清和脊髓组织中，还进行了免疫组织化学和组织病理学研究。
结果：TNF-α，IL-1β,发现第2组和第3组的IL-6水平显着增加。在第4组中，这些水平在统计学上明显下降。与其他组相比，第4组的神经运动功能测试也有显着改善。组织病理学,发现与其他组相比，第4组显示出显着减少的炎症和细胞凋亡。
结论：这项研究表明，沙芬酰胺具有抗SCI的神经保护作用，抗凋亡，和抗氧化活性。
