Abstract：
In October 2020, the first outbreaks of lumpy skin disease (LSD) in Lang Son Province, Vietnam were reported by our laboratory. The disease had rapidly spread to the South, and it was reported in 55 of 63 provinces and cities of Vietnam by the end of 2021. The most economic loss caused by this disease occurred in the north-central region in 2021 where approximately 46,788 LSD virus (LSDV) infected cattle and buffaloes have been reported and 8,976 animals have been culled. However, the information on this pathogen circulating in this region is missing. Here, we describe the molecular characterization of LSDV circulating in north-central Vietnam in 2021 and early 2022. In total, 155 LSDV samples were collected during this period and three of these samples from each province were further characterized by Sanger sequencing analysis based on three key maker genes (GPCR, RPO30, and p32). Sequence comparison and phylogenetic analysis based on GPCR, RPO30, and p32 genes indicated that LSDV strains circulating in north-central Vietnam are closely related to previously reported strains in Vietnam regions which bordered China and all LSDV strains were 100% identical. These results show the importance of continuous monitoring and characterization of circulating LSDV strains and are important for vaccine development for the control and eradication of LSD in Vietnam.
摘要：
2020年10月，郎山省首次爆发块状皮肤病(LSD)，我们的实验室报告了越南。这种疾病迅速蔓延到南方，截至2021年底，越南63个省市中的55个都有报告。该疾病造成的最大经济损失发生在2021年的中北部地区，据报道约有46,788只LSD病毒（LSDV）感染了牛和水牛，并扑杀了8,976只动物。然而,在这个地区传播的这种病原体的信息缺失。这里,我们描述了2021年和2022年初在越南中北部流通的LSDV的分子特征。总的来说,在此期间收集了155个LSDV样本，并且根据三个关键标记基因（GPCR，RPO30和p32)。基于GPCR的序列比较和系统发育分析,RPO30和p32基因表明，在越南中北部传播的LSDV菌株与先前报道的与中国接壤的越南地区的菌株密切相关，所有LSDV菌株均100％相同。这些结果表明了持续监测和表征循环LSDV毒株的重要性，并且对于在越南控制和根除LSD的疫苗开发很重要。
