Abstract：
Strain Ran72T, a novel Gram-stain-negative, obligately aerobic, non-motile, and rod-shaped bacterium, was isolated from the faeces of the rhinoceros species Ceratotherium simum. The novel bacterial strain grew optimally in Reasoner\'s 2A medium under the following conditions: 0 % (w/v) NaCl, pH 7.5, and 30 °C. Based on phylogenetic analysis using 16S rRNA gene sequencing, strain Ran72T was found to be most closely related to Chryseobacterium faecale F4T (98.4 %), Kaistella soli DKR-2T (98.0 %), and Kaistella haifensis H38T (97.4 %). A comprehensive genome-level comparison between strain Ran72T with C. faecale F4T, K. soli DKR-2T, and K. haifensis H38T revealed average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of ≤74.9, ≤19.3, and ≤78.7 %, respectively. The major fatty acids were anteiso-C15 : 0 (22.3 %), with MK-6 being the predominant respiratory quinone. The major polar lipids of strain Ran72T were phosphatidylethanolamine, four unidentified aminolipids, and two unidentified lipids. Based on our chemotaxonomic, genotypic, and phenotype characterizations, strain Ran72T was identified as representing a novel species in the genus Kaistella, for which the name Kaistella rhinocerotis sp. nov. is proposed, with the type strain Ran72T (=KACC 23136T=JCM 36038T). Based on the outcomes of our phylogenomic study, Chryseobacterium faecale should be reclassified under the genus Kaistella as Kaistella faecalis comb. nov.
摘要：
菌株Ran72T,一个新的革兰氏染色阴性，强制有氧，不活动，和杆状细菌，是从犀牛物种Ceratotheriumsimum的粪便中分离出来的。新型细菌菌株在以下条件下在Reasoner的2A培养基中最佳生长：0%（w/v）NaCl，pH7.5和30°C。基于16SrRNA基因测序的系统发育分析，发现Ran72T菌株与粪便金杆菌F4T最密切相关（98.4％），KaistellasoliDKR-2T（98.0％），和海燕H38T（97.4%）。Ran72T菌株与F4T菌株之间的综合基因组水平比较，K.soliDKR-2T，和K.haifensisH38T揭示了平均核苷酸同一性，数字DNA-DNA杂交，平均氨基酸同一性值≤74.9、≤19.3和≤78.7%，分别。主要脂肪酸是antiso-C15：0（22.3％），MK-6是主要的呼吸醌。Ran72T菌株的主要极性脂质是磷脂酰乙醇胺，四种身份不明的氨基脂类,和两种身份不明的脂质.根据我们的化学分类学，基因型，和表型表征，Ran72T菌株被鉴定为代表Kaistella属中的一种新物种，名字叫Kaistellarhinocertissp。11月。被提议，与Ran72T型菌株（=KACC23136T=JCM36038T）。根据我们的系统基因组学研究结果,粪金杆菌应重新分类为Kaistella属。11月。
