Abstract：
A negative-strand symbiotic RNA virus, tentatively named Nilaparvata lugens Bunyavirus (NLBV), was identified in the brown planthopper (BPH, Nilaparvata lugens). Phylogenetic analysis indicated that NLBV is a member of the genus Mobuvirus (family Phenuiviridae, order Bunyavirales). Analysis of virus-derived small interfering RNA suggested that antiviral immunity of BPH was successfully activated by NLBV infection. Tissue-specific investigation showed that NLBV was mainly accumulated in the fat-body of BPH adults. Moreover, NLBV was detected in eggs of viruliferous female BPHs, suggesting the possibility of vertical transmission of NLBV in BPH. Additionally, no significant differences were observed for the biological properties between NLBV-infected and NLBV-free BPHs. Finally, analysis of geographic distribution indicated that NLBV may be prevalent in Southeast Asia. This study provided a comprehensive characterization on the molecular and biological properties of a symbiotic virus in BPH, which will contribute to our understanding of the increasingly discovered RNA viruses in insects.
摘要：
一种负链共生RNA病毒,暂时命名为NilapavatalugensBunyavirus（NLBV），在褐飞虱(BPH，Nilaparvatalugens)。系统发育分析表明，NLBV是莫布病毒属（Phenuiviridae，订购Bunyavirales）。对病毒来源的小干扰RNA的分析表明，NLBV感染成功激活了BPH的抗病毒免疫。组织特异性研究表明，NLBV主要在BPH成人的脂肪体内积累。此外，在有毒力的雌性BPH的卵中检测到NLBV，表明NLBV在BPH中垂直传播的可能性。此外,NLBV感染和无NLBV的BPH之间的生物学特性没有显着差异。最后,地理分布分析表明，NLBV可能在东南亚普遍存在。本研究为BPH中共生病毒的分子和生物学特性提供了全面的表征，这将有助于我们了解昆虫中日益发现的RNA病毒。
