PDF(Pubmed)
Abstract：
Kniphofia schimperi is one of the endemic plants of Ethiopia and is widely used for the treatment of microbial infections. However, the biological and phytochemical information pertaining to this plant has not been reported so far. Anticipated by these claims, the chromatographic isolation of the CHCl3/CH3OH (1:1 v/v) extract of the roots of K. schimperi afforded five compounds, viz., knipholone (1), asphodeline (2), β-sitosterol (3), 9-pentacosenoic acid (4), and nonacosanoic acid (5). The structures of the isolated compounds were identified based on their NMR (1D and 2D) spectral data analysis and comparison with reported literature data. The crude extract and isolated compounds were evaluated for their in vitro antimicrobial activity against four bacterial strains (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Bacillus cereus) and a fungal strain (Candida albicans) using the agar disk diffusion method. The test samples showed moderate antimicrobial activity, with the highest activity observed for compound 3 (with a zone of growth inhibition of 15.5 ± 0.71 mm) against S. typhimurium.
摘要：
克氏黑曲霉是埃塞俄比亚的特有植物之一，广泛用于治疗微生物感染。然而,到目前为止，尚未报告与该植物有关的生物和植物化学信息。根据这些说法，色谱分离的CHCl3/CH3OH（1：1v/v）的根的提取物提供了五个化合物，viz.,尼福隆(1),asphodeline(2),β-谷甾醇（3），9-五碳烯酸（4），和九花生酸（5）。基于其NMR(1D和2D)光谱数据分析和与报道的文献数据的比较,鉴定分离的化合物的结构。对粗提物和分离的化合物进行了体外抗菌活性的评估，以对四种细菌菌株（大肠杆菌，鼠伤寒沙门氏菌,金黄色葡萄球菌,和蜡状芽孢杆菌）和使用琼脂圆盘扩散法的真菌菌株（白色念珠菌）。测试样品显示出中等的抗菌活性，对于化合物3(具有15.5±0.71mm的生长抑制区域)观察到的针对鼠伤寒沙门氏菌的最高活性。
