PDF(Pubmed)
Abstract：
Extracellular vesicles (EVs) can deliver various bioactive molecules among cells, making them promising diagnostic and therapeutic alternatives in diseases. Mesenchymal stem cell-derived EVs (MSC-EVs) have shown therapeutic potential similar to MSCs but with drawbacks such as lower yield, reduced biological activities, off-target effects, and shorter half-lives. Improving strategies utilizing biotechniques to pretreat MSCs and enhance the properties of released EVs, as well as modifying MSC-EVs to enhance targeting abilities and achieve controlled release, shows potential for overcoming application limitations and enhancing therapeutic effects in treating bone-related diseases. This review focuses on recent advances in functionalizing MSC-EVs to treat bone-related diseases. Firstly, we underscore the significance of MSC-EVs in facilitating crosstalk between cells within the skeletal environment. Secondly, we highlight strategies of functional-modified EVs for treating bone-related diseases. We explore the pretreatment of stem cells using various biotechniques to enhance the properties of resulting EVs, as well as diverse approaches to modify MSC-EVs for targeted delivery and controlled release. Finally, we address the challenges and opportunities for further research on MSC-EVs in bone-related diseases.
摘要：
细胞外囊泡（EV）可以在细胞之间传递各种生物活性分子，使它们成为有前途的疾病诊断和治疗替代品。间充质干细胞衍生的EV（MSC-EV）已显示出与MSC相似的治疗潜力，但具有产量较低等缺点。减少生物活性，脱靶效应,更短的半衰期。利用生物技术改进策略来预处理MSC并增强释放的EV的特性，以及修改MSC-EV以增强靶向能力并实现控释，显示了在治疗骨相关疾病中克服应用限制和增强治疗效果的潜力。本文综述了近年来MSC-EV功能化治疗骨相关疾病的研究进展。首先,我们强调MSC-EV在促进骨骼环境内细胞间串扰方面的重要性.其次,我们重点介绍了功能改良型电动汽车治疗骨相关疾病的策略.我们探索使用各种生物技术对干细胞进行预处理，以增强所得电动汽车的特性，以及修改MSC-EV以进行靶向递送和控释的多种方法。最后,我们为进一步研究骨相关疾病中的MSC-EV带来了挑战和机遇.
