PDF(Pubmed)
Abstract：
BACKGROUND: Broodiness significantly impacts poultry egg production, particularly notable in specific breeds such as the black-boned Silky, characterized by pronounced broodiness. An understanding of the alterations in ovarian signaling is essential for elucidating the mechanisms that influence broodiness. However, comparative research on the characteristics of long non-coding RNAs (lncRNAs) in the ovaries of broody chickens (BC) and high egg-laying chickens (GC) remains scant. In this investigation, we employed RNA sequencing to assess the ovarian transcriptomes, which include both lncRNAs and mRNAs, in eight Taihe Black-Bone Silky Fowls (TBsf), categorized into broody and high egg-laying groups. This study aims to provide a clearer understanding of the genetic underpinnings associated with broodiness and egg production.
RESULTS: We have identified a total of 16,444 mRNAs and 18,756 lncRNAs, of which 349 mRNAs and 651 lncRNAs exhibited significantly different expression (DE) between the BC and GC groups. Furthermore, we have identified the cis-regulated and trans-regulated target genes of differentially abundant lncRNA transcripts and have constructed an lncRNA-mRNA trans-regulated interaction network linked to ovarian follicle development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses have revealed that DE mRNAs and the target genes of DE lncRNAs are associated with pathways including neuroactive ligand-receptor interaction, CCR6 chemokine receptor binding, G-protein coupled receptor binding, cytokine-cytokine receptor interaction, and ECM-receptor interaction.
CONCLUSIONS: Our research presents a comprehensive compilation of lncRNAs and mRNAs linked to ovarian development. Additionally, it establishes a predictive interaction network involving differentially abundant lncRNAs and differentially expressed genes (DEGs) within TBsf. This significantly contributes to our understanding of the intricate interactions between lncRNAs and genes governing brooding behavior.
RESULTS: We have identified a total of 16,444 mRNAs and 18,756 lncRNAs, of which 349 mRNAs and 651 lncRNAs exhibited significantly different expression (DE) between the BC and GC groups. Furthermore, we have identified the cis-regulated and trans-regulated target genes of differentially abundant lncRNA transcripts and have constructed an lncRNA-mRNA trans-regulated interaction network linked to ovarian follicle development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analyses have revealed that DE mRNAs and the target genes of DE lncRNAs are associated with pathways including neuroactive ligand-receptor interaction, CCR6 chemokine receptor binding, G-protein coupled receptor binding, cytokine-cytokine receptor interaction, and ECM-receptor interaction.
CONCLUSIONS: Our research presents a comprehensive compilation of lncRNAs and mRNAs linked to ovarian development. Additionally, it establishes a predictive interaction network involving differentially abundant lncRNAs and differentially expressed genes (DEGs) within TBsf. This significantly contributes to our understanding of the intricate interactions between lncRNAs and genes governing brooding behavior.
摘要：
背景：育蛋显著影响禽蛋产量，特别值得注意的是，在特定的品种，如黑骨丝，以明显的亲熟为特征。了解卵巢信号传导的变化对于阐明影响亲熟的机制至关重要。然而,关于育卵鸡（BC）和高产蛋鸡（GC）卵巢中长非编码RNA（lncRNA）特征的比较研究仍然很少。在这次调查中,我们使用RNA测序来评估卵巢转录组，包括lncRNAs和mRNAs，在八只太和乌骨丝鸡（TBsf）中，分为育卵组和高产卵组。这项研究旨在更清楚地了解与育卵和产蛋相关的遗传基础。
结果：我们总共鉴定了16,444个mRNAs和18,756个lncRNAs，其中349个mRNAs和651个lncRNAs在BC和GC组之间表现出显著不同的表达(DE)。此外，我们已经确定了差异丰富的lncRNA转录本的顺式调节和反式调节的靶基因，并构建了一个与卵泡发育相关的lncRNA-mRNA反式调节相互作用网络。基因本体论（GO）和京都基因和基因组百科全书（KEGG）注释分析显示，DEmRNAs和DElncRNAs的靶基因与包括神经活性配体-受体相互作用在内的通路相关。CCR6趋化因子受体结合，G蛋白偶联受体结合，细胞因子-细胞因子受体相互作用，和ECM-受体相互作用。
结论：我们的研究提出了与卵巢发育相关的lncRNAs和mRNAs的综合汇编。此外,它建立了一个预测性相互作用网络，涉及TBsf中差异丰富的lncRNAs和差异表达基因（DEG）。这极大地有助于我们理解lncRNAs和控制沉思行为的基因之间的复杂相互作用。
结果：我们总共鉴定了16,444个mRNAs和18,756个lncRNAs，其中349个mRNAs和651个lncRNAs在BC和GC组之间表现出显著不同的表达(DE)。此外，我们已经确定了差异丰富的lncRNA转录本的顺式调节和反式调节的靶基因，并构建了一个与卵泡发育相关的lncRNA-mRNA反式调节相互作用网络。基因本体论（GO）和京都基因和基因组百科全书（KEGG）注释分析显示，DEmRNAs和DElncRNAs的靶基因与包括神经活性配体-受体相互作用在内的通路相关。CCR6趋化因子受体结合，G蛋白偶联受体结合，细胞因子-细胞因子受体相互作用，和ECM-受体相互作用。
结论：我们的研究提出了与卵巢发育相关的lncRNAs和mRNAs的综合汇编。此外,它建立了一个预测性相互作用网络，涉及TBsf中差异丰富的lncRNAs和差异表达基因（DEG）。这极大地有助于我们理解lncRNAs和控制沉思行为的基因之间的复杂相互作用。
