Abstract：
High-salt content in food waste (FW) affects its resource utilization during biotransformation. In this study, adaptive laboratory evolution (ALE), gene editing, and artificial consortia were performed out to improve the salt-tolerance of Bacillus amyloliquefaciens for producing lipopeptide under FW and seawater. High-salt stress significantly decreased lipopeptide production in the B. amyloliquefaciens HM618 and ALE strains. The total lipopeptide production in the recombinant B. amyloliquefaciens HM-4KSMSO after overexpressing the ion transportor gene ktrA and proline transporter gene opuE and replacing the promoter of gene mrp was 1.34 times higher than that in the strain HM618 in medium containing 30 g/L NaCl. Lipopeptide production under salt-tolerant consortia containing two strains (HM-4KSMSO and Corynebacterium glutamicum) and three-strains (HM-4KSMSO, salt-tolerant C. glutamicum, and Yarrowia lipolytica) was 1.81- and 2.28-fold higher than that under pure culture in a medium containing FW or both FW and seawater, respectively. These findings provide a new strategy for using high-salt FW and seawater to produce value-added chemicals.
摘要：
食物垃圾（FW）中的高盐含量会影响其生物转化过程中的资源化利用。在这项研究中,适应性实验室进化(ALE)，基因编辑,并进行人工聚生体，以提高解淀粉芽孢杆菌在FW和海水下生产脂肽的耐盐性。高盐胁迫显着降低了解淀粉芽孢杆菌HM618和ALE菌株中脂肽的产生。在过表达离子转运基因ktrA和脯氨酸转运蛋白基因opuE并替换基因mrp的启动子后，重组解淀粉芽孢杆菌HM-4KSMSO中的总脂肽产量是菌株HM618在含30g/LNaCl的培养基中的1.34倍。在含有两个菌株（HM-4KSMSO和谷氨酸棒杆菌）和三个菌株（HM-4KSMSO，耐盐谷氨酸棒杆菌，和Yarrowialipolypolitica）比在含有FW或FW和海水的培养基中纯培养下高1.81-和2.28倍，分别。这些发现为使用高盐FW和海水生产增值化学品提供了新的策略。
