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Abstract：
OBJECTIVE: Dental erosion is a chemical-mechanical process that leads to the loss of dental hard tissues. This study aimed to investigate the effect of pomegranate juice on the enamel.
METHODS: Enamel blocks were randomly divided into three groups: deionized water, cola, and pomegranate juice. The blocks were immersed in the solutions four times a day for 14 days, and stored in artificial saliva for the remaining period. The surface hardness was measured on days 7 and 14. The surface structures of the demineralized blocks were observed via scanning electron microscopy (SEM), and the depth of demineralization was observed via confocal laser scanning microscopy (CLSM). The pH, calcium, and phosphorus levels of the three solutions were analyzed.
RESULTS: The microhardness values of the blocks in the pomegranate juice and cola groups decreased with the increase in the demineralization time. The blocks in the pomegranate juice group exhibited large fractures in the enamel column, whereas those in the cola group had pitted enamels with destruction of the interstitial enamel column. Compared with cola group, fluorescent penetration increased in pomegranate juice (P < 0.01). The pH of cola (2.32 ± 0.09) was lower than that of pomegranate juice (3.16 ± 0.16). Furthermore, the calcium content in pomegranate juice was significantly higher than that in cola (P < 0.01). Alternatively, the concentration of phosphorous in cola was significantly higher than that in pomegranate juice (P < 0.01).
CONCLUSIONS: These findings indicate that pomegranate juice can cause enamel demineralization with an erosive potential comparable to that of cola.
METHODS: Enamel blocks were randomly divided into three groups: deionized water, cola, and pomegranate juice. The blocks were immersed in the solutions four times a day for 14 days, and stored in artificial saliva for the remaining period. The surface hardness was measured on days 7 and 14. The surface structures of the demineralized blocks were observed via scanning electron microscopy (SEM), and the depth of demineralization was observed via confocal laser scanning microscopy (CLSM). The pH, calcium, and phosphorus levels of the three solutions were analyzed.
RESULTS: The microhardness values of the blocks in the pomegranate juice and cola groups decreased with the increase in the demineralization time. The blocks in the pomegranate juice group exhibited large fractures in the enamel column, whereas those in the cola group had pitted enamels with destruction of the interstitial enamel column. Compared with cola group, fluorescent penetration increased in pomegranate juice (P < 0.01). The pH of cola (2.32 ± 0.09) was lower than that of pomegranate juice (3.16 ± 0.16). Furthermore, the calcium content in pomegranate juice was significantly higher than that in cola (P < 0.01). Alternatively, the concentration of phosphorous in cola was significantly higher than that in pomegranate juice (P < 0.01).
CONCLUSIONS: These findings indicate that pomegranate juice can cause enamel demineralization with an erosive potential comparable to that of cola.
摘要：
目的：牙齿侵蚀是一种化学机械过程，会导致牙齿硬组织的损失。本研究旨在研究石榴汁对牙釉质的影响。
方法：牙釉质块随机分为三组：去离子水，可乐,还有石榴汁.将这些块浸入溶液中，每天四次，持续14天，并在剩余的时间内储存在人工唾液中。在第7天和第14天测量表面硬度。通过扫描电子显微镜（SEM）观察去矿质块的表面结构，通过共聚焦激光扫描显微镜（CLSM）观察脱矿深度。pH值,钙，并对三种溶液的磷水平进行了分析。
结果：石榴汁和可乐组的块的显微硬度值随着脱矿时间的增加而降低。石榴汁组中的块在釉质柱中显示出较大的骨折，而可乐组的釉质凹陷，间质釉质柱被破坏。与可乐组相比,石榴汁的荧光渗透增加(P<0.01)。可乐的pH（2.32±0.09）低于石榴汁的pH（3.16±0.16）。此外，石榴汁中钙含量明显高于可乐（P<0.01）。或者，可乐中的磷浓度明显高于石榴汁（P<0.01）。
结论：这些研究结果表明，石榴汁可导致牙釉质脱矿，其侵蚀潜力与可乐相当。
方法：牙釉质块随机分为三组：去离子水，可乐,还有石榴汁.将这些块浸入溶液中，每天四次，持续14天，并在剩余的时间内储存在人工唾液中。在第7天和第14天测量表面硬度。通过扫描电子显微镜（SEM）观察去矿质块的表面结构，通过共聚焦激光扫描显微镜（CLSM）观察脱矿深度。pH值,钙，并对三种溶液的磷水平进行了分析。
结果：石榴汁和可乐组的块的显微硬度值随着脱矿时间的增加而降低。石榴汁组中的块在釉质柱中显示出较大的骨折，而可乐组的釉质凹陷，间质釉质柱被破坏。与可乐组相比,石榴汁的荧光渗透增加(P<0.01)。可乐的pH（2.32±0.09）低于石榴汁的pH（3.16±0.16）。此外，石榴汁中钙含量明显高于可乐（P<0.01）。或者，可乐中的磷浓度明显高于石榴汁（P<0.01）。
结论：这些研究结果表明，石榴汁可导致牙釉质脱矿，其侵蚀潜力与可乐相当。
