PDF(Pubmed)
Abstract：
BACKGROUND: Various animal models have been used to explore the pathogenesis of choledochal cysts (CCs), but with little convincing results. Current surgical techniques can achieve satisfactory outcomes for treatment of CCs. Consequently, recent studies have focused more on clinical issues rather than basic research. Therefore, we need appropriate animal models to further basic research.
OBJECTIVE: To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of CCs.
METHODS: Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group, sham surgical group, or control group. A rat model of CC was established by partial ligation of the bile duct. The reliability of the model was confirmed by measurements of serum biochemical indices, morphology of common bile ducts of the rats as well as molecular biology experiments in rat and human tissues.
RESULTS: Dilation classified as mild (diameter, ≥ 1 mm to < 3 mm), moderate (≥ 3 mm to < 10 mm), and severe (≥ 10 mm) was observed in 17, 17, and 2 rats in the surgical group, respectively, while no dilation was observed in the control and sham surgical groups. Serum levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery, while direct bilirubin, total bilirubin, and gamma-glutamyltransferase were further increased 14 d after surgery. Most of the biochemical indices gradually decreased to normal ranges 28 d after surgery. The protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC tissues.
CONCLUSIONS: The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC, which may contribute to investigating the pathogenesis of CC.
OBJECTIVE: To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of CCs.
METHODS: Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group, sham surgical group, or control group. A rat model of CC was established by partial ligation of the bile duct. The reliability of the model was confirmed by measurements of serum biochemical indices, morphology of common bile ducts of the rats as well as molecular biology experiments in rat and human tissues.
RESULTS: Dilation classified as mild (diameter, ≥ 1 mm to < 3 mm), moderate (≥ 3 mm to < 10 mm), and severe (≥ 10 mm) was observed in 17, 17, and 2 rats in the surgical group, respectively, while no dilation was observed in the control and sham surgical groups. Serum levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery, while direct bilirubin, total bilirubin, and gamma-glutamyltransferase were further increased 14 d after surgery. Most of the biochemical indices gradually decreased to normal ranges 28 d after surgery. The protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC tissues.
CONCLUSIONS: The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC, which may contribute to investigating the pathogenesis of CC.
摘要：
背景：已使用各种动物模型来探索胆总管囊肿（CC）的发病机理，但几乎没有令人信服的结果。目前的手术技术可以获得令人满意的治疗效果。因此,最近的研究更多地集中在临床问题上，而不是基础研究。因此,我们需要适当的动物模型来进一步进行基础研究。
目的：建立合适的动物模型，为研究CCs的发病机制提供依据。
方法：将84只无特定病原体雌性Sprague-Dawley大鼠随机分配到手术组，假手术组,或对照组。通过胆管部分结扎建立大鼠CC模型。通过测量血清生化指标证实了模型的可靠性，大鼠胆总管的形态学以及大鼠和人体组织的分子生物学实验。
结果：扩张分类为轻度（直径，≥1mm至<3mm),中等(≥3mm至<10mm),在手术组的17、17和2只大鼠中观察到严重（≥10mm），分别,而对照组和假手术组未观察到扩张。血清丙氨酸转氨酶水平,天冬氨酸转氨酶,总胆红素,直接胆红素,和总胆汁酸在手术后7d明显高于对照组,而直接胆红素，总胆红素,术后14d，γ-谷氨酰转移酶进一步升高。大部分生化指标在术后28d逐渐下降至正常范围。大鼠模型中信号转导和转录激活因子3的蛋白表达趋势与人CC组织一致。
结论：幼年大鼠胆管部分结扎模型可在形态学上模拟囊性或梭形CC，这可能有助于研究CC的发病机制。
目的：建立合适的动物模型，为研究CCs的发病机制提供依据。
方法：将84只无特定病原体雌性Sprague-Dawley大鼠随机分配到手术组，假手术组,或对照组。通过胆管部分结扎建立大鼠CC模型。通过测量血清生化指标证实了模型的可靠性，大鼠胆总管的形态学以及大鼠和人体组织的分子生物学实验。
结果：扩张分类为轻度（直径，≥1mm至<3mm),中等(≥3mm至<10mm),在手术组的17、17和2只大鼠中观察到严重（≥10mm），分别,而对照组和假手术组未观察到扩张。血清丙氨酸转氨酶水平,天冬氨酸转氨酶,总胆红素,直接胆红素,和总胆汁酸在手术后7d明显高于对照组,而直接胆红素，总胆红素,术后14d，γ-谷氨酰转移酶进一步升高。大部分生化指标在术后28d逐渐下降至正常范围。大鼠模型中信号转导和转录激活因子3的蛋白表达趋势与人CC组织一致。
结论：幼年大鼠胆管部分结扎模型可在形态学上模拟囊性或梭形CC，这可能有助于研究CC的发病机制。
