PDF(Pubmed)
Abstract：
UNASSIGNED: Pakistan is an agricultural country; most of its income is based on livestock rearing. The increasing prevalence of tick-borne pathogens among animals may affect the animal production and livelihood of owners, which eventually derange the economy of a country.
UNASSIGNED: To further comprehend TBPs, 213 ticks were collected from different animals, including ruminants, pets, and poultry. After molecular and phylogenetic analysis identification, ticks were managed into different pools based on their species level (Hyalomma anatolicum = 80, Rhipicephalus microplus = 35, Hyalomma scupense = 23, Rhipicephalus turanicus = 70, and Rhipicephalus sanguineus = 5).
UNASSIGNED: After tick species identification, further molecular PCR amplification was carried out to screen out the pathogens for the presence of Theileria, Rickettsia, Anaplasma, and enzootic nasal tumor virus (ENTV). The following pathogens were detected: 11 (5.16%) for Anaplasma, 1 (0.47%) for Rickettsia, and 9 (4.23%) for Theileria. Nevertheless, other TBPs that had not been reported so far in Pakistan 3 (1.41%), were positive for enzootic nasal tumor virus (ENTV). Besides, phylogenetic analysis of the enzootic nasal tumor virus (ENTV) strain confirmed its resemblance to the Chinese strain, while Anaplasma has comparability with Pakistan and China, Rickettsia with Pakistan, China, and Iran, and Theileria with India, South Africa, United States, Japan, and Spain.
UNASSIGNED: This study reveals that there is a considerably wider range of TBPs held in Pakistan that take in various contagious zoonotic pathogens than was previously thought. This information advances TBP epidemiology and will contribute to upgrade future control measure.
UNASSIGNED: To further comprehend TBPs, 213 ticks were collected from different animals, including ruminants, pets, and poultry. After molecular and phylogenetic analysis identification, ticks were managed into different pools based on their species level (Hyalomma anatolicum = 80, Rhipicephalus microplus = 35, Hyalomma scupense = 23, Rhipicephalus turanicus = 70, and Rhipicephalus sanguineus = 5).
UNASSIGNED: After tick species identification, further molecular PCR amplification was carried out to screen out the pathogens for the presence of Theileria, Rickettsia, Anaplasma, and enzootic nasal tumor virus (ENTV). The following pathogens were detected: 11 (5.16%) for Anaplasma, 1 (0.47%) for Rickettsia, and 9 (4.23%) for Theileria. Nevertheless, other TBPs that had not been reported so far in Pakistan 3 (1.41%), were positive for enzootic nasal tumor virus (ENTV). Besides, phylogenetic analysis of the enzootic nasal tumor virus (ENTV) strain confirmed its resemblance to the Chinese strain, while Anaplasma has comparability with Pakistan and China, Rickettsia with Pakistan, China, and Iran, and Theileria with India, South Africa, United States, Japan, and Spain.
UNASSIGNED: This study reveals that there is a considerably wider range of TBPs held in Pakistan that take in various contagious zoonotic pathogens than was previously thought. This information advances TBP epidemiology and will contribute to upgrade future control measure.
摘要：
■巴基斯坦是一个农业国家；它的大部分收入是靠牲畜饲养。蜱传病原体在动物中的日益流行可能会影响动物的生产和主人的生计，最终会破坏一个国家的经济。
■为了进一步理解TBP，从不同的动物中收集了213只蜱，包括反刍动物,宠物,和家禽。经过分子和系统发育分析鉴定，根据蜱的物种水平将蜱分为不同的池（Hyalommaanatolicum=80，Rhipicephalusmicroplus=35，Hyalommascupense=23，Rhipicephalusturanicus=70，Rhipicephalussanguineus=5）。
■在蜱物种鉴定后，进行了进一步的分子PCR扩增，以筛选出存在Theileria的病原体，立克次体,解脲脲,和地方性鼻瘤病毒（ENTV）。检测到以下病原体:11例(5.16%)为无性体,立克次体为1（0.47%），和9（4.23％）为Theileria。然而，巴基斯坦迄今未报告的其他TBP3（1.41%），对地方性鼻瘤病毒（ENTV）呈阳性。此外,口蹄疫鼻瘤病毒（ENTV）菌株的系统发育分析证实了其与中国菌株的相似性，Anaplasma与巴基斯坦和中国具有可比性，立克次体与巴基斯坦，中国,伊朗,西里利亚和印度，南非,美国,Japan,和西班牙。
■这项研究表明，巴基斯坦持有的TBP吸收各种传染性人畜共患病病原体的范围比以前认为的要广泛得多。这些信息促进了TBP流行病学，并将有助于升级未来的控制措施。
■为了进一步理解TBP，从不同的动物中收集了213只蜱，包括反刍动物,宠物,和家禽。经过分子和系统发育分析鉴定，根据蜱的物种水平将蜱分为不同的池（Hyalommaanatolicum=80，Rhipicephalusmicroplus=35，Hyalommascupense=23，Rhipicephalusturanicus=70，Rhipicephalussanguineus=5）。
■在蜱物种鉴定后，进行了进一步的分子PCR扩增，以筛选出存在Theileria的病原体，立克次体,解脲脲,和地方性鼻瘤病毒（ENTV）。检测到以下病原体:11例(5.16%)为无性体,立克次体为1（0.47%），和9（4.23％）为Theileria。然而，巴基斯坦迄今未报告的其他TBP3（1.41%），对地方性鼻瘤病毒（ENTV）呈阳性。此外,口蹄疫鼻瘤病毒（ENTV）菌株的系统发育分析证实了其与中国菌株的相似性，Anaplasma与巴基斯坦和中国具有可比性，立克次体与巴基斯坦，中国,伊朗,西里利亚和印度，南非,美国,Japan,和西班牙。
■这项研究表明，巴基斯坦持有的TBP吸收各种传染性人畜共患病病原体的范围比以前认为的要广泛得多。这些信息促进了TBP流行病学，并将有助于升级未来的控制措施。
