PDF(Pubmed)
Abstract：
Antimicrobial photoinactivation (API) has shown some promise in potentially treating different nosocomial bacterial infections, however, its application on staphylococci, especially other than Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) species is still limited. Although S. aureus is a well-known and important nosocomial pathogen, several other species of the genus, particularly coagulase-negative Staphylococcus (CNS) species such as Staphylococcus epidermidis and Staphylococcus saprophyticus, can also cause healthcare-associated infections and foodborne intoxications. CNS are often involved in resilient biofilm formation on medical devices and can cause infections in patients with compromised immune systems or those undergoing invasive procedures. In this study, the effects of chlorophyllin and riboflavin-mediated API on S. epidermidis and S. saprophyticus planktonic cells and biofilm are demonstrated for the first time. Based on the residual growth determination and metabolic reduction ability changes, higher inactivating efficiency of chlorophyllin-mediated API was determined against the planktonic cells of both tested species of bacteria and against S. saprophyticus biofilm. Some insights on whether aqueous solutions of riboflavin and chlorophyllin, when illuminated with optimal exciting wavelength (440 nm and 402 nm, respectively) generate O2-•, are also provided in this work.
摘要：
抗微生物光灭活（API）在潜在治疗不同的医院细菌感染方面显示出一些希望，然而,它在葡萄球菌上的应用，尤其是除金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌(MRSA)外,种类仍然有限.尽管金黄色葡萄球菌是众所周知的和重要的医院病原体,该属的其他几种，特别是凝固酶阴性葡萄球菌（CNS），如表皮葡萄球菌和腐生葡萄球菌，还可能导致医疗保健相关的感染和食源性中毒。CNS通常参与医疗装置上的弹性生物膜形成,并且可以在免疫系统受损的患者或经历侵入性程序的患者中引起感染。在这项研究中,首次证明了叶绿素和核黄素介导的API对表皮葡萄球菌和腐生链球菌浮游细胞和生物膜的影响。根据残留生长测定和代谢还原能力的变化，叶绿素介导的API对受试细菌的浮游细胞和腐生链球菌生物膜均具有较高的灭活效率。关于核黄素和叶绿素水溶液的一些见解，当用最佳激发波长（440nm和402nm，分别)生成O2-·，在这项工作中也提供了。
