PDF(Pubmed)
Abstract：
UNASSIGNED: A combined microbial and cytological examination of uterine samples is the main diagnostic method for endometritis in mares. This study aimed to describe a procedure for using the same uterine cytobrush (CB) for both bacteriological and cytological evaluation.
UNASSIGNED: The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet.
UNASSIGNED: The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results.
UNASSIGNED: The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts.
UNASSIGNED: The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet.
UNASSIGNED: The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results.
UNASSIGNED: The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts.
摘要：
■对子宫样本进行微生物和细胞学联合检查是诊断母马子宫内膜炎的主要方法。这项研究旨在描述使用相同的子宫细胞刷（CB）进行细菌学和细胞学评估的程序。
■该程序包括在收集后立即将CB滚动到灭菌的玻璃载玻片上，然后转移到无菌盐水溶液中。在实验1中，对10匹母马的棉签（CS）和CB或颗粒的细菌学结果进行了比较；在实验2中，对28匹母马的不同加工方法之间的细菌学和细胞学结果进行了比较；在其他6匹母马中，仅对CB进行细胞学处理，调查颗粒细胞性低的原因。
■评估了CB和CS培养结果之间的一致性，并对CB的不同处理方法获得的细胞学数据进行了比较。发现CB和CS微生物学结果之间的完美一致性。所描述的程序使细胞学的诊断涂片有用。此外，CB尖端和用于运输的盐溶液的播种产生了准确的细菌学结果。
■本研究中描述的使用CB进行细胞学和细菌学分析的方案可用于子宫内膜炎的诊断。为了最大限度地提高诊断样本质量，细胞学载玻片必须在现场精心准备，以保持细胞完整性并最大程度地减少伪影。
■该程序包括在收集后立即将CB滚动到灭菌的玻璃载玻片上，然后转移到无菌盐水溶液中。在实验1中，对10匹母马的棉签（CS）和CB或颗粒的细菌学结果进行了比较；在实验2中，对28匹母马的不同加工方法之间的细菌学和细胞学结果进行了比较；在其他6匹母马中，仅对CB进行细胞学处理，调查颗粒细胞性低的原因。
■评估了CB和CS培养结果之间的一致性，并对CB的不同处理方法获得的细胞学数据进行了比较。发现CB和CS微生物学结果之间的完美一致性。所描述的程序使细胞学的诊断涂片有用。此外，CB尖端和用于运输的盐溶液的播种产生了准确的细菌学结果。
■本研究中描述的使用CB进行细胞学和细菌学分析的方案可用于子宫内膜炎的诊断。为了最大限度地提高诊断样本质量，细胞学载玻片必须在现场精心准备，以保持细胞完整性并最大程度地减少伪影。
