Abstract：
Two pairs of cyclohexene amide alkaloid enantiomers were obtained from the root of Piper nigrum. Their plane structures were established by NMR and HRESIMS spectra. The absolute configurations of 1a/1b and 2a/2b were determined by the comparison between the experimental and calculated electronic circular dichroism (ECD) spectra. All identified compounds were tested for inhibitory effects on acetylcholinesterase (AChE) in vitro. Notably, compounds 1b and 2b showed strong inhibitory effects on AChE and the interaction between proteins and compounds was discussed by molecular docking studies.
摘要：
从黑胡椒的根中获得了两对环己烯酰胺生物碱对映体。通过NMR和HRESIMS谱确定了它们的平面结构。通过实验和计算的电子圆二色性（ECD）光谱之间的比较确定1a/1b和2a/2b的绝对构型。在体外测试所有鉴定的化合物对乙酰胆碱酯酶(AChE)的抑制作用。值得注意的是,化合物1b和2b对AChE有很强的抑制作用，并通过分子对接研究讨论了蛋白质与化合物之间的相互作用。
