Abstract：
Thyroid cancer (THCA) is the most prevalent endocrine tumor, and its incidence continues to increase every year. However, the processes underlying the aggressive progression of thyroid cancer are unknown. We concentrated on the prognostic and biological importance of thyroid cancer cuproptosis-related genes in this investigation. Genomic and clinical data were obtained from the UCSC XENA website, and cuproptosis-related genes were obtained from the FerrDb website. We performed differential expression analysis and Cox regression analysis to identify possible predictive targets associated with thyroid cancer prognosis. To assess the role of CDKN2A in thyroid cancer and the ability to predict prognosis on the basis of the CDKN2A expression level, we performed immunohistochemical staining, survival analysis, immunological analysis, functional analysis, and clinical analysis with respect to CDKN2A gene expression. CDKN2A expression levels were found to be inversely correlated with thyroid cancer prognosis. Higher levels of CDKN2A expression were associated with higher T, N, and clinicopathological stage and more residual tumor cells. Through univariate and multivariate Cox regression analyses, the CDKN2A expression level was shown to be linked with thyroid cancer patients\' overall survival (OS). Moreover, we discovered that CDKN2A expression was linked to a dysfunctional tumor immune microenvironment. The study shows that CDKN2A, a cuproptosis-related gene, can be used as a prognostic marker for thyroid cancer.
摘要：
甲状腺癌（THCA）是最常见的内分泌肿瘤，发病率逐年上升。然而,甲状腺癌侵袭性进展的潜在过程尚不清楚.在这项研究中，我们专注于甲状腺癌角化相关基因的预后和生物学重要性。基因组和临床数据来自UCSCXENA网站，并从FerrDb网站获得角化相关基因。我们进行了差异表达分析和Cox回归分析，以确定与甲状腺癌预后相关的可能预测目标。根据CDKN2A的表达水平评估CDKN2A在甲状腺癌中的作用和预测预后的能力。我们进行了免疫组织化学染色，生存分析,免疫学分析，功能分析，以及CDKN2A基因表达的临床分析。CDKN2A表达水平与甲状腺癌预后呈负相关。较高水平的CDKN2A表达与较高的T相关。N,和临床病理分期和较多残留肿瘤细胞。通过单变量和多变量Cox回归分析，CDKN2A表达水平与甲状腺癌患者总生存期(OS)相关.此外，我们发现CDKN2A的表达与功能失调的肿瘤免疫微环境有关.研究表明，CDKN2A，一个与角化相关的基因，可作为甲状腺癌的预后标志物。
