PDF(Pubmed)
Abstract：
OBJECTIVE: The objective of this study was to characterize extracellular vesicles (EVs) in plasma and synovial fluid obtained from horses with and without naturally occurring post-traumatic osteoarthritis (PTOA).
METHODS: EVs were isolated from plasma and synovial fluid from horses with (n = 6) and without (n = 6) PTOA.
METHODS: Plasma and synovial fluid EVs were characterized with respect to quantity, size, and surface markers. Small RNA sequencing was performed, and differentially expressed microRNAs (miRNAs) underwent bioinformatic analysis to identify putative targets and to explore potential associations with specific biological processes.
RESULTS: Plasma and synovial fluid samples from horses with PTOA had a significantly higher proportion of exosomes and a lower proportion of microvesicles compared to horses without PTOA. Small RNA sequencing revealed several differentially expressed miRNAs, including miR-144, miR-219-3p, and miR-199a-3l in plasma and miR-199a-3p, miR-214, and miR-9094 in synovial fluid EVs. Bioinformatics analysis of the differentially expressed miRNAs highlighted their potential role in fibrosis, differentiation of chondrocytes, apoptosis, and inflammation pathways in PTOA.
CONCLUSIONS: We have identified dynamic molecular changes in the small noncoding signatures of plasma and synovial fluid EVs in horses with naturally occurring PTOA. These findings could serve to identify promising biomarkers in the pathogenesis of PTOA, to facilitate the development of targeted therapies, and to aid in establishing appropriate translational models of PTOA.
METHODS: EVs were isolated from plasma and synovial fluid from horses with (n = 6) and without (n = 6) PTOA.
METHODS: Plasma and synovial fluid EVs were characterized with respect to quantity, size, and surface markers. Small RNA sequencing was performed, and differentially expressed microRNAs (miRNAs) underwent bioinformatic analysis to identify putative targets and to explore potential associations with specific biological processes.
RESULTS: Plasma and synovial fluid samples from horses with PTOA had a significantly higher proportion of exosomes and a lower proportion of microvesicles compared to horses without PTOA. Small RNA sequencing revealed several differentially expressed miRNAs, including miR-144, miR-219-3p, and miR-199a-3l in plasma and miR-199a-3p, miR-214, and miR-9094 in synovial fluid EVs. Bioinformatics analysis of the differentially expressed miRNAs highlighted their potential role in fibrosis, differentiation of chondrocytes, apoptosis, and inflammation pathways in PTOA.
CONCLUSIONS: We have identified dynamic molecular changes in the small noncoding signatures of plasma and synovial fluid EVs in horses with naturally occurring PTOA. These findings could serve to identify promising biomarkers in the pathogenesis of PTOA, to facilitate the development of targeted therapies, and to aid in establishing appropriate translational models of PTOA.
摘要：
目的：这项研究的目的是表征从有或没有自然发生的创伤后骨关节炎（PTOA）的马获得的血浆和滑液中的细胞外囊泡（EV）。
方法：从有（n=6）和没有（n=6）PTOA的马的血浆和滑液中分离出EV。
方法：对血浆和滑液EV的数量进行表征，尺寸,和表面标记。进行小RNA测序，和差异表达的microRNAs（miRNAs）进行了生物信息学分析，以确定推定的靶标并探索与特定生物过程的潜在关联。
结果：与没有PTOA的马相比，来自具有PTOA的马的血浆和滑液样品具有明显更高的外泌体比例和更低的微泡比例。小RNA测序揭示了几种差异表达的miRNA，包括miR-144，miR-219-3p，血浆中的miR-199a-3l和miR-199a-3p，滑液EV中的miR-214和miR-9094。差异表达miRNA的生物信息学分析强调了它们在纤维化中的潜在作用，软骨细胞的分化，凋亡，和PTOA中的炎症途径。
结论：我们已经确定了具有天然存在的PTOA的马的血浆和滑液EV的小的非编码特征的动态分子变化。这些发现可能有助于鉴定PTOA发病机制中的有希望的生物标志物，为了促进靶向治疗的发展，并帮助建立适当的PTOA翻译模型。
方法：从有（n=6）和没有（n=6）PTOA的马的血浆和滑液中分离出EV。
方法：对血浆和滑液EV的数量进行表征，尺寸,和表面标记。进行小RNA测序，和差异表达的microRNAs（miRNAs）进行了生物信息学分析，以确定推定的靶标并探索与特定生物过程的潜在关联。
结果：与没有PTOA的马相比，来自具有PTOA的马的血浆和滑液样品具有明显更高的外泌体比例和更低的微泡比例。小RNA测序揭示了几种差异表达的miRNA，包括miR-144，miR-219-3p，血浆中的miR-199a-3l和miR-199a-3p，滑液EV中的miR-214和miR-9094。差异表达miRNA的生物信息学分析强调了它们在纤维化中的潜在作用，软骨细胞的分化，凋亡，和PTOA中的炎症途径。
结论：我们已经确定了具有天然存在的PTOA的马的血浆和滑液EV的小的非编码特征的动态分子变化。这些发现可能有助于鉴定PTOA发病机制中的有希望的生物标志物，为了促进靶向治疗的发展，并帮助建立适当的PTOA翻译模型。
