PDF(Pubmed)
Abstract：
Under iron (Fe)-limited conditions, plants have developed strategies for acquiring this essential micronutrient. Several Fe sources have been studied as potential fertilizers, with Fe synthetic chelates being the most used to prevent and correct Fe chlorosis in crops. The determination of the activity of the Fe chelate reductase (FCR) enzyme has long been described in the literature to understand the efficiency of Strategy I plants in acquiring Fe from fertilizers under deficient conditions. Other experiments have focused on the translocation of Fe to the plant to define the effectiveness of Fe fertilizers. Yet, both assays are relevant in knowing the capacity of a novel Fe source and other compounds alleviating Fe chlorosis in Strategy I plants. This work reviews the methodologies that are used in FCR assays to evaluate novel Fe fertilizers, including the factors modulating the results obtained for FCR assay activity, such as the Fe substrate, the Fe level during the growing period and during the FCR assay, the pH, the choice of an in vivo or in vitro method, and the plant species. A discussion of the benefits of the concurrence of FCR and Fe uptake assays is then presented alongside a proposed methodology for assessing the effectiveness of Fe fertilizers, emphasizing the importance of understanding chemical and physiological plant interactions. This methodology unifies key factors that modify FCR activity and combines these with the use of the 57Fe tracer to enhance our comprehension of the efficacy of Fe-based fertilizers\' effectiveness in alleviating Fe chlorosis. This comprehensive approach not only contributes to the fundamental understanding of Fe-deficient Strategy I plants but also establishes a robust method for determining the efficiency of novel sources for correcting Fe deficiency in plants.
摘要：
在铁（Fe）限制的条件下，植物已经制定了获取这种必需微量营养素的策略。已经研究了几种铁源作为潜在的肥料，铁合成螯合物最常用于预防和纠正作物中的铁黄化。在文献中早已描述了Fe螯合还原酶（FCR）活性的测定，以了解策略I植物在缺乏条件下从肥料中获得Fe的效率。其他实验集中于Fe向植物的转运，以确定Fe肥料的有效性。然而，两种测定都与了解新型Fe源和其他化合物减轻策略I植物中Fe黄化的能力有关。这项工作回顾了FCR测定中用于评估新型Fe肥料的方法，包括调节FCR测定活性结果的因素，如Fe衬底，生长期和FCR测定期间的铁水平，pH值,体内或体外方法的选择，和植物物种。然后，与评估Fe肥料有效性的拟议方法一起讨论了FCR和Fe吸收测定的同时存在的好处。强调理解化学和生理植物相互作用的重要性。该方法统一了改变FCR活性的关键因素，并将这些因素与57Fe示踪剂的使用相结合，以增强我们对Fe基肥料减轻Fe萎黄病的功效的理解。这种全面的方法不仅有助于对缺铁策略I植物的基本理解，而且还建立了一种可靠的方法来确定新来源纠正植物缺铁的效率。
