Abstract：
Cyantraniliprole (CY), an anthranilic diamide insecticide widely used in grape farming for controlling various sucking pests, poses ecological concerns, particularly when applied as soil drenching due to the formation of more toxic and persistent metabolites. This study established the dissipation and degradation mechanisms of CY in grape rhizosphere soil using high-resolution Orbitrap-LC/MS analysis. The persistence of CY residues beyond 60 days was observed, with dissipation following biphasic first + first-order kinetics and a half-life of 15 to 21 days. The degradation mechanism of CY in the soil was elucidated, with identified metabolites such as IN-J9Z38, IN-JCZ38, IN-N7B69, and IN-QKV54. Notably, CY was found to predominantly convert to the highly persistent metabolite IN-J9Z38, raising environmental concerns. The impact of CY residues on soil enzyme activity was investigated, revealing a negative effect on dehydrogenase, alkaline phosphatase, and acid phosphatase activity, indicating significant implications for phosphorous mineralization and soil health. Furthermore, bacterial isolates were obtained from CY-enriched soil, with five isolates (CY3, CY4, CY9, CY11, and CY20) demonstrating substantial degradation potential, ranging from 66 to 92% of CY residues. These results indicate that the identified bacteria hold potential for commercial use in addressing pesticide residue contamination in soil through bioremediation techniques.
摘要：
Cyantraniliprole(CY),一种广泛用于葡萄种植的邻氨基二酰胺杀虫剂，用于控制各种吸吮害虫，带来了生态问题，特别是当由于形成更多的毒性和持久性代谢物而被用作土壤浸水时。本研究利用高分辨率Orbitrap-LC/MS分析建立了CY在葡萄根际土壤中的耗散和降解机制。观察到CY残留物的持久性超过60天，耗散遵循双相一级+一级动力学，半衰期为15至21天。阐明了CY在土壤中的降解机理，与鉴定的代谢物如IN-J9Z38、IN-JCZ38、IN-N7B69和IN-QKV54。值得注意的是,发现CY主要转化为高持久性代谢物IN-J9Z38，引起了环境问题。研究了CY残留对土壤酶活性的影响,揭示了对脱氢酶的负面影响，碱性磷酸酶,和酸性磷酸酶活性，表明对磷矿化和土壤健康具有重要意义。此外，细菌分离株是从富含CY的土壤中获得的，五个分离株（CY3，CY4，CY9，CY11和CY20）显示出很大的降解潜力，范围从66％到92％的CY残基。这些结果表明，已鉴定的细菌具有通过生物修复技术解决土壤中农药残留污染的商业用途的潜力。
