PDF(Pubmed)
Abstract：
While functioning in the oral cavity, denture soft linings (SL) are exposed to contact with the microbiota. Dentures can offer perfect conditions for the multiplication of pathogenic yeast-like fungi, resulting in rapid colonisation of the surface of the materials used. In vitro experiments have also shown that yeast may penetrate SL. This may lead to changes in their initially beneficial functional properties. The aim of this work was to investigate the effect of three months of exposure to a Candida albicans suspension on the mechanical properties of SL material and its bond strength to the denture base polymer, and to additionally verify previous reports of penetration using a different methodology. Specimens of the SL material used were incubated for 30, 60 and 90 days in a suspension of Candida albicans strain (ATCC 10231). Their shore A hardness, tensile strength, and bond strength to acrylic resin were tested. The colonization of the surface and penetration on fractured specimens were analysed with scanning electron and inverted fluorescence microscopes. Exposure to yeast did not affect the mechanical properties. The surfaces of the samples were colonised, especially in crystallized structures of the medium; however, the penetration of hyphae and blastospores into the material was not observed.
摘要：
在口腔中起作用时，义齿软衬(SL)暴露于与微生物群接触。假牙可以为致病性酵母样真菌的繁殖提供完美的条件，导致所用材料表面的快速定殖。体外实验还表明酵母可以穿透SL。这可能导致它们最初有益的功能特性的变化。这项工作的目的是研究三个月暴露于白色念珠菌悬浮液对SL材料的机械性能及其与义齿基托聚合物的粘合强度的影响，并使用不同的方法额外验证以前的渗透报告。将所使用的SL材料的样品在白色念珠菌菌株(ATCC10231)的悬浮液中孵育30、60和90天。它们的肖氏A硬度，抗拉强度,并测试了与丙烯酸树脂的粘结强度。用扫描电子和倒置荧光显微镜分析了表面的定植和在断裂标本上的渗透。暴露于酵母不会影响机械性能。样品的表面被定殖，尤其是在介质的结晶结构中；然而，没有观察到菌丝和小孢子渗透到材料中。
