PDF(Pubmed)
Abstract：
UNASSIGNED: The limited existing knowledge regarding resistance to antimicrobial peptides (AMPs) is hindering their broad utilization. The aim of this study is to enhance the understanding of AMP resistance, a pivotal factor in the exploration of alternative drug development in response to the escalating challenge of antibiotic resistance.
UNASSIGNED: We utilized metagenomic functional selection to analyze genes resistant to AMPs, with a specific focus on the microbiota in soil and the human gut. Through a combination of experimental methods and bioinformatics analyses, our investigation delved into the possibilities of the evolution of resistance to AMPs, as well as the transfer or interchange of resistance genes among the environment, the human body, and pathogens. Additionally, we examined the cross-resistance between AMPs and evaluated interactions among AMPs and conventional antibiotics.
UNASSIGNED: The presence of AMP resistance, including various resistance mechanisms, was observed in both soil and the human gut microbiota, as indicated by our findings. Significantly, the study underscored the facile evolution of AMP resistance and the potential for gene sharing or exchange among different environments. Notably, cross-resistance among AMPs was identified as a phenomenon, while cross-resistance between AMPs and antibiotics was found to be relatively infrequent.
UNASSIGNED: The results of our study highlight the significance of taking a cautious stance when considering the extensive application of AMPs. It is imperative to thoroughly assess potential resistance risks, with a particular focus on the development of resistance to AMPs across diverse domains. A comprehensive grasp of these aspects is essential for making well-informed decisions and ensuring the responsible utilization of AMPs in the ongoing fight against antibiotic resistance.
UNASSIGNED: We utilized metagenomic functional selection to analyze genes resistant to AMPs, with a specific focus on the microbiota in soil and the human gut. Through a combination of experimental methods and bioinformatics analyses, our investigation delved into the possibilities of the evolution of resistance to AMPs, as well as the transfer or interchange of resistance genes among the environment, the human body, and pathogens. Additionally, we examined the cross-resistance between AMPs and evaluated interactions among AMPs and conventional antibiotics.
UNASSIGNED: The presence of AMP resistance, including various resistance mechanisms, was observed in both soil and the human gut microbiota, as indicated by our findings. Significantly, the study underscored the facile evolution of AMP resistance and the potential for gene sharing or exchange among different environments. Notably, cross-resistance among AMPs was identified as a phenomenon, while cross-resistance between AMPs and antibiotics was found to be relatively infrequent.
UNASSIGNED: The results of our study highlight the significance of taking a cautious stance when considering the extensive application of AMPs. It is imperative to thoroughly assess potential resistance risks, with a particular focus on the development of resistance to AMPs across diverse domains. A comprehensive grasp of these aspects is essential for making well-informed decisions and ensuring the responsible utilization of AMPs in the ongoing fight against antibiotic resistance.
摘要：
■关于抗微生物肽(AMPs)抗性的有限的现有知识阻碍了它们的广泛利用。本研究的目的是提高对AMP抗性的认识，探索替代药物开发以应对不断升级的抗生素耐药性挑战的关键因素。
■我们利用宏基因组功能选择来分析抗AMPs的基因，特别关注土壤和人体肠道中的微生物群。通过实验方法和生物信息学分析相结合，我们的调查深入研究了抗性激素进化的可能性，以及抗性基因在环境中的转移或互换，人体,和病原体。此外,我们检查了AMPs之间的交叉耐药性,并评估了AMPs与常规抗生素之间的相互作用.
■AMP电阻的存在，包括各种抵抗机制，在土壤和人类肠道微生物群中都观察到，正如我们的发现所表明的。重要的是,该研究强调了AMP抗性的轻松进化以及不同环境之间基因共享或交换的潜力。值得注意的是,AMP之间的交叉抗性被确定为一种现象，而发现AMPs和抗生素之间的交叉耐药相对罕见。
■我们的研究结果凸显了在考虑AMPs的广泛应用时采取谨慎立场的重要性。必须彻底评估潜在的抵抗风险，特别关注跨不同领域的AMPs抗性的发展。全面掌握这些方面对于做出明智的决定并确保在持续的对抗抗生素耐药性的斗争中负责任地利用AMP至关重要。
■我们利用宏基因组功能选择来分析抗AMPs的基因，特别关注土壤和人体肠道中的微生物群。通过实验方法和生物信息学分析相结合，我们的调查深入研究了抗性激素进化的可能性，以及抗性基因在环境中的转移或互换，人体,和病原体。此外,我们检查了AMPs之间的交叉耐药性,并评估了AMPs与常规抗生素之间的相互作用.
■AMP电阻的存在，包括各种抵抗机制，在土壤和人类肠道微生物群中都观察到，正如我们的发现所表明的。重要的是,该研究强调了AMP抗性的轻松进化以及不同环境之间基因共享或交换的潜力。值得注意的是,AMP之间的交叉抗性被确定为一种现象，而发现AMPs和抗生素之间的交叉耐药相对罕见。
■我们的研究结果凸显了在考虑AMPs的广泛应用时采取谨慎立场的重要性。必须彻底评估潜在的抵抗风险，特别关注跨不同领域的AMPs抗性的发展。全面掌握这些方面对于做出明智的决定并确保在持续的对抗抗生素耐药性的斗争中负责任地利用AMP至关重要。
