PDF(Pubmed)
Abstract：
Scabies is considered an external parasite notorious for its high prevalence causing severe and contagious skin lesions in humans and animals worldwide. This study has introduced a medicine to treat dogs infested with scabies (variants of Demodex, Sarcoptes, Psoroptes, Otodectes, etc.). The present study offers a no-side-effect herbal formulation to treat dogs infested with scabies. Unlike oral and injectable medicines, which take the form of an ointment and are topically applied on-site, this medicinal formulation can be easily used without concerns over its side effects or consumption dosages. This medicinal formulation requires no skin rinsing due to its herbal and high skin absorption properties, as recovery may take less than a month with a maximum of two times of application. To carry out the experiment, 25 sick dogs with various breeds and ages suspected of scabies were gathered. Following accurate morphological examinations of all the samples, a deep skin chip of the lesion site was provided, which was examined by a microscope. Then, 13 dogs (Mix, Terrier, Pug, Husky, Spitz) were infested with Demodex scabies and 12 dogs (Pittbull, Mix, Shih Tzu, Terrier, Boxer, Setter) with Sarcoptic scabies. The prepared product was topically administered at a constant 2% dosage to the bodies of all the samples. To prepare the ointment, 1 g of Borax (Na2B4O7·10H2O) was first dissolved in 35 g deionized water and heated to 70°C. Then, 45 g of liquid paraffin (CnH2n+2) was mixed with 1 g of Carvacrol (C10H14O) and 1 g of geranium (C10H18O) and stirred well to become a phase. Later, 17 g of the melted beeswax (C15H31COOC30H61) was added to the liquid paraffin compound. In the end, the aqueous phase was added to the oil phase, and the mixture process immediately began in one direction with a glass stirrer and continued until the product cooled down. Essential oils (EO) was obtained by steam distillation of fresh Thyme and Rose-Acented Geranium in a stainless steel distillation apparatus (alembic) for 3 h. The main components of the essential oils used in the formulation were performed using a Hewlett-Packard GC system interfaced with a mass spectrometer equipped with an HP5-MS capillary column (30 m, 0.32 mm, 0.25 µm film thicknesses). For GC-MS detection, electron ionization with ionization energy of 70 eV was used. To examine the presence of scabies, weekly skin sampling was performed, and the treatment continued until 30 days, when no skin chip of the scabies was noted. The findings revealed that the formulation developed no side effects and removed the daily use, as it could be administered once or twice a week. Also, complete recovery of scabies in all the breeds was found to be less than a month at most. This medicinal formulationcan be easily used without concerns over its side effects or consumption dosages. This study introduced a herbal formulation with effective herbal ingredients without any side effects to treat the sarcoptes and demodex parasites; unlike other chemical compounds, this medicinal formulation has no side effects, while some other formulations could develop side effects.
摘要：
镰刀菌被认为是一种外部寄生虫，因其高患病率而在全世界的人类和动物中引起严重和传染性的皮肤损伤而臭名昭著。这项研究引入了一种药物来治疗患有sc疮的狗（Demodex的变体，Sarcoptes,Psoroptes,Otodectes,等。).本研究提供了一种无副作用的草药配方来治疗患有sc疮的狗。与口服和注射药物不同，采取软膏的形式，并在现场局部施用，这种药物制剂可以很容易地使用，而不必担心其副作用或消费剂量。由于其草药和高皮肤吸收特性，这种药物制剂不需要皮肤冲洗，因为恢复可能需要不到一个月的时间，最多申请两次。为了进行实验，收集了25只不同品种和年龄的病犬，怀疑有sc疮。在对所有样品进行准确的形态学检查后，提供了病变部位的深层皮肤芯片，用显微镜检查。然后，13只狗（混合，梗,帕格,赫斯基，Spitz）感染了蠕形螨sc疮和12只狗（Pittbull，混合,施子,梗,拳击手,二传手）与沙棘。将制备的产品以恒定的2%剂量局部给予所有样品的身体。准备药膏,首先将1g硼砂(Na2B4O7·10H2O)溶解在35g去离子水中并加热至70℃。然后，将45g液体石蜡(CnH2n+2)与1g香芹酚(C10H14O)和1g天竺葵(C10H18O)混合并充分搅拌以形成相。稍后，将17g熔化的蜂蜡(C15H31COOC30H61)加入到液体石蜡化合物中。最后,将水相加入到油相中，用玻璃搅拌器在一个方向上立即开始混合过程,并继续直到产品冷却。精油（EO）是通过在不锈钢蒸馏装置（alembic）中蒸汽蒸馏新鲜百里香和玫瑰红天竺葵3小时而获得的。配方中使用的精油的主要成分是使用Hewlett-PackardGC系统与配备HP5-MS毛细管柱（30m，0.32mm,0.25µm薄膜厚度)。对于GC-MS检测，使用70eV的电离能的电子电离。为了检查是否有镰刀,每周进行皮肤采样，治疗持续到30天，当没有皮肤切片的sc疮被发现。研究结果表明，该制剂没有副作用，消除了日常使用，因为它可以每周给药一次或两次。此外，发现所有品种的sc疮完全恢复最多不到一个月。这种药用配方可以很容易地使用，而无需担心其副作用或消费剂量。这项研究引入了一种草药配方，具有有效的草药成分，没有任何副作用，可以治疗化石和蠕形螨寄生虫;与其他化合物不同，这种药物配方没有副作用，而其他一些制剂可能会产生副作用。
