Abstract：
This work explores the potential of three hypersaline native microalgae strains from Oklahoma, Geitlerinema carotinosum, Pseudanabaena sp., and Picochlorum oklahomensis, for simultaneous treatment of flowback (FW) and produced wastewater (PW) and the production of algal biomass. The quality of wastewater before and after treatment with these microalgae strains was evaluated and a characterization of algal biomass in terms of moisture, volatile matter, fixed carbon, and ash contents was assessed. The experimental results indicated how all the microalgae strains were able to grow in both FW and PW, revealing their potential for wastewater treatment. Although algal biomass production was limited by nutrient availability both in PW and FW, a maximum biomass concentration higher than 1.35 g L-1 were achieved by the three strains in two of the PWs and one of the FWs tested, with Pseudanabaena sp. reaching nearly 2 g L-1. Interestingly, higher specific growth rates were obtained by the two cyanobacteria strains G. carotinosum and Pseudanabaena sp. when cultivated in both PW and FW, compared to P. oklahomensis. The harvested algal biomass contained a significant amount of energy, even though it was significantly reduced by the very high salt content. The energy content fell within the recommended range of 16-17 MJ kg-1 for biomass as feedstock for biofuels. The algal treatment resulted in the complete removal of ammonia from the wastewater and a significant reduction in contaminants, such as nitrate, phosphate, boron, and micronutrients like zinc, manganese, and iron.
摘要：
这项工作探索了来自俄克拉荷马州的三种高盐天然微藻菌株的潜力，Geitlerinema胡萝卜素，Pseudanabaenasp.,还有秋葵皮草,用于同时处理返排（FW）和产生的废水（PW）以及藻类生物质的生产。评估了用这些微藻菌株处理前后的废水质量，并根据水分对藻类生物量进行了表征，挥发性物质，固定碳,并评估灰分含量。实验结果表明，所有微藻菌株都能够在FW和PW中生长，揭示了它们在废水处理方面的潜力。尽管藻类生物量的生产受到PW和FW中养分利用率的限制，通过测试的两个PWs和一个FWs中的三个菌株实现了高于1.35gL-1的最大生物量浓度，与伪anabaenasp.达到近2gL-1。有趣的是,两种蓝细菌菌株G.carotinosum和Pseudanabaenasp。获得了更高的比生长速率。当在PW和FW中种植时，与P.oklahomensis相比。收获的藻类生物质含有大量的能量，尽管它被非常高的盐含量显著降低。作为生物燃料原料的生物质的能量含量在16-17MJkg-1的推荐范围内。藻类处理导致废水中氨的完全去除和污染物的显著减少，如硝酸盐，磷酸盐,硼，和微量营养素如锌，锰，和铁。
