Abstract：
Tuberculosis (TB) is one of the contagious diseases caused by M. tuberculosis (MTB) bacteria. Prompt diagnosis is one of the active solutions to control the spread of this infection. Besides, a targeted, specific and non-complex diagnosis can prove promising in this type of epidemic. This study was designed to compare the efficiencies of a diagnosis by Ziehl-Neelsen staining (ZN) and by the polymerase chain reaction (PCR) technique. Samples presented smear-positive pulmonary TB were subjected to Chromosomal restriction fragment length polymorphism of IS6110 (IS6110-RFLP) for fingerprinting profile determination. The results showed that out of 100 sputum samples of suspected case, 53 were positive. Numbers of positive individuals for tuberculosis obtained by the different diagnostic techniques, to know, (ZN staining; culture and PCR) were respectively: 6, 25 and 22. Chromosomal RFLP fingerprinting profile revealed the presence of five different genotypes obtained from seven tested isolates. These results suggest that molecular techniques are alternative tool for fast and specific diagnosis of pulmonary MTB from sputum.
摘要：
结核病（TB）是由结核分枝杆菌（MTB）细菌引起的传染病之一。及时诊断是控制这种感染传播的积极解决方案之一。此外,有针对性的,在这种类型的流行病中，特异性和非复杂性的诊断可能被证明是有希望的。本研究旨在比较Ziehl-Neelsen染色（ZN）和聚合酶链反应（PCR）技术的诊断效率。对涂片阳性肺结核的样品进行IS6110（IS6110-RFLP）的染色体限制性片段长度多态性，以进行指纹图谱测定。结果显示,在100份疑似病例的痰液样本中,53为阳性。通过不同的诊断技术获得的结核病阳性个体数量，要知道,(ZN染色；培养和PCR)分别为：6、25和22。染色体RFLP指纹图谱显示存在从七个测试的分离物中获得的五种不同的基因型。这些结果表明，分子技术是快速，特异性诊断痰中肺部MTB的替代工具。
