关键词: CLARITY Tissue clearing electrophoresis imaging

Mesh : Electrophoresis / instrumentation methods

来  源:   DOI:10.1080/03091902.2024.2336497

Abstract:
Electrophoretic tissue clearing has been a commonly used laboratory method since the early twentieth century. Infrastructure for standard procedures has yet to be formed. In particular, control of the heat produced by electrophoresis, the voltage applied to the electrodes, the resistance, and the speed of liquid circulation create difficulty for researchers. We aimed to develop a compact organ electrophoresis system that enables the researcher to have easy, rapid, and inexpensive working opportunities. The system includes an electronic control unit, a liquid tank, a temperature control unit, and an electrophoresis chamber. The control unit software can keep the system stable by using information on temperature and circulation rate received through the sensors using the feedback principle. Corrosion and particle collection are reduced to a minimum as platinum wires are used for electrophoresis electrodes. A temperature control unit can heat and cool via a liquid tank base. The CORES is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. It assures efficient, rapid, and consistent tissue clearing. The system was stable with 72 h of continuous operation. Patent applications and trial version studies for introducing the system to researchers are still in progress.
摘要:
自20世纪初以来,电泳技术已成为一种常用的实验室方法。标准程序的基础结构尚未形成。特别是,控制电泳产生的热量,施加到电极上的电压,抵抗,液体循环的速度给研究人员带来了困难。我们旨在开发一种紧凑的器官电泳系统,使研究人员能够轻松,快速,廉价的工作机会。该系统包括一个电子控制单元,一个液体罐,温度控制单元,和电泳室。控制单元软件可以通过使用反馈原理通过传感器接收的温度和循环速率的信息来保持系统稳定。腐蚀和颗粒收集减少到最低限度,因为铂线用于电泳电极。温度控制单元可以通过液体罐底座加热和冷却。CORES是一体化的,易于使用的解决方案,用于电泳组织清理。它确保高效,快速,和一致的组织清理。连续运行72h,系统稳定。向研究人员介绍该系统的专利申请和试用版研究仍在进行中。
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