Abstract：
This study explored the effects of different vitamin B5 (VB5) levels on intestinal growth and function of weaned piglets. Twenty-one piglets (7.20 ± 1.11 kg) were included in a 28-day feeding trial with three treatments, including 0 mg/kg (L-VB5), 10 mg/kg (Control) and 50 mg/kg (H-VB5) of VB5 supplement. The results showed that: Large intestine weight/body weight was the highest in H-VB5 group, Control and H-VB5 groups had significantly higher villus height and villus height/crypt depth than the L-VB5 in the ileum (p < .05). Goblet cells (ileal crypt) and endocrine cells (ileal villus) significantly increased in Control and H-VB5 (p < .05). The H-VB5 group exhibited significantly higher levels of ki67 and crypt depth in the cecum and colon, colonic goblet cells and endocrine cells were both rising considerably (p < .05). Isobutyric acid and isovaleric acid were significantly reduced in the H-VB5 group (p < .05), and there was a decreasing trend in butyric acid (p = .073). At the genus level, the relative abundance of harmful bacteria such as Clostridium_Sensu_Structo_1 Strecto_1, Terrisporbacter and Streptococcus decreased significantly and the relative abundance of beneficial bacteria Turicibacter increased significantly in H-VB5 group (p < .05). Overall, the addition of 50 mg/kg VB5 primarily enhanced the morphological structure, cell proliferation and differentiation of the ileum, cecum and colon. It also had a significant impact on the gut microbiota and short-chain fatty acids.
摘要：
本研究探讨了不同维生素B5(VB5)水平对断奶仔猪肠道生长和功能的影响。21只仔猪(7.20±1.11kg)参加了一项为期28天的三次饲喂试验,包括0mg/kg（L-VB5），10mg/kg(对照)和50mg/kg(H-VB5)的VB5补充剂。结果表明:H-VB5组大肠重/体重最高,对照组和H-VB5组的绒毛高度和绒毛高度/隐窝深度明显高于回肠中的L-VB5（p<0.05）。对照和H-VB5中的杯状细胞(回肠隐窝)和内分泌细胞(回肠绒毛)显著增加(p<.05)。H-VB5组在盲肠和结肠中表现出显著较高的ki67水平和隐窝深度,结肠杯状细胞和内分泌细胞均显著升高(p<0.05)。异丁酸和异戊酸在H-VB5组中显著降低(p<0.05),丁酸呈下降趋势(p=.073)。在属一级，H-VB5组有害细菌如梭状芽孢杆菌Strecto_1，Terrisporter杆菌和链球菌的相对丰度显着降低，有益细菌Turicibacter的相对丰度显着增加（p<0.05）。总的来说,50mg/kgVB5的添加主要增强了形态结构，回肠的细胞增殖和分化，盲肠和结肠。它还对肠道微生物群和短链脂肪酸产生重大影响。
