Abstract：
Alarin is a newly discovered neuropeptide that belongs to the galanin peptide family with a wide range of bioactivity in the nervous system. Its function in the brain\'s autonomic areas has been studied, and it has been reported that alarin is involved in the regulation of excitability in hypothalamic neurons. Its role in the regulation of excitability in the hippocampus, however, is unknown. In this study, we investigated if alarin induced any synchronous discharges or epileptiform activity, and if it had any effect on already initiated epileptiform discharges. We used thick acute horizontal hippocampal slices obtained from 30‑ to 35‑day‑old rats. Extracellular field potential recordings were evaluated in the CA1 region of the hippocampus. Our data demonstrated that, alarin application did not result in any epileptiform activity or abnormal discharges. 4‑aminopyridine was applied to induce epileptiform activity in the slices. We found that alarin increased the frequency of interictal‑like events and the mean power of local field potentials in the CA1 region of the hippocampus, which was induced by 4‑aminopyridine. These results demonstrated for the first time that alarin has a modulatory effect on synchronized neuronal discharges and showed the contribution of the neuropeptide alarin to epilepsy‑like conditions.
摘要：
Alarin是一种新发现的神经肽，属于galanin肽家族，在神经系统中具有广泛的生物活性。它在大脑自主神经区域的功能已被研究，据报道，alarin参与调节下丘脑神经元的兴奋性。它在调节海马兴奋性中的作用，然而,是未知的。在这项研究中,我们调查了Alarin是否诱导了任何同步放电或癫痫样活动，以及它是否对已经开始的癫痫样放电有任何影响。我们使用了从30至35天大的大鼠获得的厚的急性水平海马切片。在海马CA1区评估细胞外场电位记录。我们的数据表明,alarin的应用没有导致任何癫痫样活动或异常放电。应用4-氨基吡啶诱导切片中的癫痫样活性。我们发现alarin增加了海马CA1区的间期样事件的频率和局部场电位的平均功率，由4-氨基吡啶诱导。这些结果首次证明了alarin对同步神经元放电具有调节作用，并显示了神经肽alarin对癫痫样疾病的贡献。
