Abstract：
Sweet tea is a functional herbal tea with anti-inflammatory, anti-diabetic, and other effects, in which phloridzin and trilobatin are two functional compounds. However, the current methods for their quantification are time-consuming, costly, and environmentally unfriendly. In this paper, we propose a rapid method that integrates online pressurized liquid extraction and high-performance liquid chromatography featuring a superficially porous column for fast separation. Moreover, we employ an equal absorption wavelength method to eliminate using multiple standard solutions and relative calibration factors. Our verification process corroborated the technique\'s selectivity, accuracy, precision, linearity, and detection limitations. Separately, our methodology demonstrated excellent analytical efficiency, cost-effectiveness, and environmental friendliness. Practical application using six distinct batches of sweet tea samples yielded results in congruence with the external standard method. The analytical rate of this technique is up to over 18 times faster than traditional methods, and organic solvent consumption has been reduced to less than 1.5 mL. Therefore, this method provides a valuable way to achieve quality control and green analysis of sweet tea and other herbal teas.
摘要：
甜茶是一种具有抗炎作用的功能性凉茶，抗糖尿病，和其他影响，其中根皮苷和三叶抑素是两种功能化合物。然而,目前的量化方法很耗时，昂贵的,对环境不友好。在本文中,我们提出了一种快速的方法，该方法集成了在线加压液体萃取和高效液相色谱，其特征是表面多孔柱用于快速分离。此外，我们采用等吸收波长方法来消除使用多个标准溶液和相对校准因子。我们的验证过程证实了该技术的选择性，准确度,精度,线性度和检测限制。分别,我们的方法表现出卓越的分析效率，成本效益，和环境友好。使用六个不同批次的甜茶样品的实际应用产生了与外标方法一致的结果。这种技术的分析速度比传统方法快18倍以上，有机溶剂消耗已减少到小于1.5mL。因此,该方法为实现甜茶和其他凉茶的质量控制和绿色分析提供了有价值的方法。
