Abstract：
Fungi have different genetic expression abilities and biosynthetic pathways under different cultivation conditions, which can produce various secondary metabolites. The \"one strain many compounds\" strategy is used to activate silent biosynthetic genes of fungi to produce various compounds, which is an effective method. In order to discover various new compounds in the edible fungus Pholiota nameko, a fermentation strategy involving precursor feeding and enzyme inhibitor addition has been employed. A new illudane sesquiterpene (1), along with one known indole diterpenoid alkaloid, cladosporine A (2) were isolated from the extracts of liquid culture of P. nameko. The new compound was identified by combination of 1D and 2D NMR, MS, optical rotation, and ECD calculations. We conducted experiments on the cytotoxicity of all isolated compounds on three cancer cell lines, but we did not observe any significant cytotoxicity (IC50 > 40 μM).
摘要：
真菌在不同的培养条件下具有不同的遗传表达能力和生物合成途径,可以产生各种次生代谢产物。“一株多化合物”策略用于激活真菌的沉默生物合成基因以产生各种化合物，这是一种有效的方法。为了在食用菌Pholiotanameko中发现各种新化合物，已经采用了涉及前体进料和酶抑制剂添加的发酵策略。一种新的伊卢丹倍半萜(1)，连同一种已知的吲哚二萜生物碱，从P.nameko的液体培养物的提取物中分离出克多孢菌素A（2）。通过1D和2DNMR的组合鉴定了新化合物，MS,旋光,和ECD计算。我们对所有分离的化合物对三种癌细胞系的细胞毒性进行了实验，但我们没有观察到任何显著的细胞毒性(IC50>40μM)。
