PDF(Pubmed)
Abstract：
Genetic alterations in the TP63 (GenBank: NC_000003.12, ID: 8626) and CCR5 (receptor 5 chemokine co-receptor) (GenBank: NC_000003.12, ID: 1234) genes may increase the risk of developing breast cancer. The aim of this study was to investigate the probable involvement of polymorphisms rs17506395 in the TP63 (tumour protein 63) gene and the CCR5Δ32 mutation in the occurrence of breast cancer in Burkina Faso. This case-control study included 72 patients and 72 controls. Genotyping of SNP rs17506395 (TP63) was performed by polymerase chain reaction-restriction fragment length polymorphism, and genotyping of the CCR5Δ32 mutation was performed by allele-specific oligonucleotide polymerase chain reaction. For SNP rs17506395 (TP63), the genotypic frequencies of wild-type homozygotes (TT) and heterozygotes (TG) were, respectively, 27.72 and 72.22% in cases and 36.11 and 63.89% in controls. No mutated homozygotes (GG) were observed. For the CCR5Δ32 mutation, the genotypic frequencies of wild-type homozygotes (WT/WT) and heterozygotes (WT/Δ32) were 87.5 and 13.5%, respectively, in the cases and 89.29 and 10.71%, respectively, in the controls. No mutated homozygotes (Δ32/Δ32) were observed. None of the polymorphisms rs17506395 of the TP63 gene (OR = 1.47, 95% CI = 0.69-3.17, P = 0.284) and the CCR5Δ32 mutation (OR = 1.32, 95% CI = 0.46-3.77; P = 0.79) were associated with the occurrence of breast cancer in this study.
摘要：
TP63（GenBank：NC_000003.12，ID：8626）和CCR5（受体5趋化因子共受体）（GenBank：NC_000003.12，ID：1234）基因的遗传改变可能会增加患乳腺癌的风险。这项研究的目的是调查TP63（肿瘤蛋白63）基因rs17506395多态性和CCR5Δ32突变在布基纳法索乳腺癌发生中的可能参与。这项病例对照研究包括72名患者和72名对照。通过聚合酶链反应-限制性片段长度多态性进行SNPrs17506395（TP63）的基因分型，并通过等位基因特异性寡核苷酸聚合酶链反应对CCR5Δ32突变进行基因分型。对于SNPrs17506395(TP63)，野生型纯合子（TT）和杂合子（TG）的基因型频率分别为，分别,病例为27.72％和72.22％，对照组为36.11％和63.89％。没有观察到突变的纯合子(GG)。对于CCR5Δ32突变，野生型纯合子(WT/WT)和杂合子(WT/Δ32)的基因型频率分别为87.5%和13.5%,分别,在89.29和10.71％的情况下，分别,在控制中。没有观察到突变的纯合子(Δ32/Δ32)。TP63基因rs17506395多态性(OR=1.47,95%CI=0.69～3.17,P=0.284)和CCR5Δ32突变(OR=1.32,95%CI=0.46～3.77,P=0.79)均与乳腺癌的发生无相关性。
